Search
Search Results
-
Human mast cells induce osteoclastogenesis through cell surface RANKL
ObjectivesWe employed the co-culture of CD34 + stem cell-derived human mast cells (HMC) and human monocyte-derived osteoclast precursors to evaluate...
-
Integrated single-cell chromatin and transcriptomic analyses of human scalp identify gene-regulatory programs and critical cell types for hair and skin diseases
Genome-wide association studies have identified many loci associated with hair and skin disease, but identification of causal variants requires...
-
SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell–derived human neurons
Sarbecoviruses such as SARS and SARS-CoV-2 have been responsible for two major outbreaks in humans, the latter resulting in a global pandemic. While...
-
Pirfenidone affects human cardiac fibroblast proliferation and cell cycle activity in 2D cultures and engineered connective tissues
The anti-fibrotic drug pirfenidone (PFD) is currently in clinical testing for the treatment of heart failure with preserved ejection fraction;...
-
Aging modifies endometrial dendritic cell function and unconventional double negative T cells in the human genital mucosa
BackgroundImmune function in the genital mucosa balances reproduction with protection against pathogens. As women age, genital infections, and...
-
Regulon active landscape reveals cell development and functional state changes of human primary osteoblasts in vivo
BackgroundWhile transcription factor (TF) regulation is known to play an important role in osteoblast development, differentiation, and bone...
-
Deciphering Oligodendrocyte Lineages in the Human Fetal Central Nervous System Using Single-Cell RNA Sequencing
Oligodendrocytes form myelin sheaths and wrap axons of neurons to facilitate various crucial neurological functions. Oligodendrocyte progenitor cells...
-
N-Terminomics/TAILS of Human Tumor Biopsies and Cancer Cell Lines
Proteases serve essential roles in numerous biological processes and signaling cascades by cleaving their substrates in a restricted manner or via...
-
Comparative transcriptomic and phenotypic analysis of induced pluripotent stem cell hepatocyte-like cells and primary human hepatocytes
Primary human hepatocytes (PHHs) are used extensively for in vitro liver cultures to study hepatic functions. However, limited availability and...
-
Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era
This chapter describes the core procedures that we have developed over the last two decades to isolate routinely the microglia from postmortem human...
-
Alveolar epithelial-like cell differentiation in a dynamic bioreactor: a promising 3D-approach for the high-throughput generation of lung cell types from human induced pluripotent stem cells
PurposeHuman induced pluripotent stem cell (hiPSC)-derived lung cell types such as alveolar epithelial cells are promising for toxicological and...
-
Human mesenchymal stem cell secretomes: Factors affecting profiling and challenges in clinical application
The promising field of regenerative medicine is thrilling as it can repair and restore organs for various debilitating diseases. Mesenchymal stem...
-
CD4+ T cells produce IFN-I to license cDC1s for induction of cytotoxic T-cell activity in human tumors
CD4 + T cells can "help” or "license” conventional type 1 dendritic cells (cDC1s) to induce CD8 + cytotoxic T lymphocyte (CTL) anticancer responses, as...
-
Human T-cell lymphotropic virus type 1 (HTLV-1) grip on T-cells: investigating the viral tapestry of activation
IntroductionHuman T-cell Lymphotropic virus type 1 (HTLV-1) belongs to retroviridae which is connected to two major diseases, including...
-
Elastin-derived peptides (EDPs) as a potential pro-malignancy factor in human leukemia cell lines
AbstractThe extracellular matrix (ECM) is currently considered to be an important factor influencing the migration and progression of cancer cells....
-
Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles
BackgroundSingle-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene...
-
Mechanisms of metabolic stress induced cell death of human oligodendrocytes: relevance for progressive multiple sclerosis
Oligodendrocyte (OL) injury and loss are central features of evolving lesions in multiple sclerosis. Potential causative mechanisms of OL loss...
-
Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis
BackgroundCurrently there are no established fertility preservation options for pre-pubertal boys facing cancer treatment. Granulocyte-colony...
-
Transcriptomics of Human Brain Tissue in Parkinson’s Disease: a Comparison of Bulk and Single-cell RNA Sequencing
Parkinson’s disease (PD) is a chronic and progressive neurodegenerative disease leading to motor dysfunction and, in some cases, dementia....
