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Effect of water salinity on immature performance and lifespan of adult Asian tiger mosquito
BackgroundAedes albopictus (Skuse, 1894) is a vector for pathogens like dengue, chikungunya, and Zika viruses. Its adaptive capacity enables...
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Albumin-based nanoparticles as contrast medium for MRI: vascular imaging, tissue and cell interactions, and pharmacokinetics of second-generation nanoparticles
This multidisciplinary study examined the pharmacokinetics of nanoparticles based on albumin-DTPA-gadolinium chelates, testing the hypothesis that...
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The Role of Reactive Oxygen Species and Calcium Ions in Implementing the Stress-Protective Effect of γ-Aminobutyric Acid on Wheat Seedlings Under Heat Stress Conditions
γ-aminobutyric acid (GABA) is considered a molecule that combines the properties of a stress metabolite and a signaling molecule. At the same time,...
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Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells for Viable Biobanking
Biorepositories of fresh frozen and formalin-fixed paraffin-embedded tissues have been foundational to many molecular cancer research studies....
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Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes
Organoids with region-specific architecture could facilitate the repair of injuries of the central nervous system. Here we show that human astrocytes...
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PhysioCell — a Novel, Bio-relevant Dissolution Apparatus: Hydrodynamic Conditions and Factors Influencing the Dissolution Dynamics
The physiologically relevant dissolution apparatuses simulate various aspects of gastrointestinal physiology and help to understand and predict the in...
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Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes
BackgroundToday, cosmetic products are very popular with both men and women to improve their appearance and increase their social acceptability.
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Adapting field-mosquito collection techniques in a perspective of near-infrared spectroscopy implementation
BackgroundNear-infrared spectroscopy (NIRS) has the potential to be a useful tool for assessing key entomological parameters of malaria-transmitting...
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Luminescence-Based, Low- and Medium-Throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage
Schistosomiasis is one of the major parasitic diseases with more than 200 million people infected worldwide every year. Praziquantel is the drug of...
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Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion in the Culture Medium
Hairy root (HR) cultures have proved to be an interesting platform for the production of biopharmaceutical proteins mainly for their intrinsic...
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A Novel Azathioprine Polysaccharide Nanoparticle Enhances the Efficacy of Systemic Lupus Erythematosus Therapy
Systemic lupus erythematosus (SLE) patients will suffer from some level of discomfort as the disease progresses due to involvement with the...
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Pearls and Pitfalls of Isolating Rat OPCs for In Vitro Culture with Different Methods
There are several in vitro models to study the biology of oligodendrocyte progenitor cells (OPCs). The use of models based on induced pluripotent...
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Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders
Human-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited...
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Next-generation sequencing reveals mitogenome diversity in plasma extracellular vesicles from colorectal cancer patients
BackgroundRecent reports have demonstrated that the entire mitochondrial genome can be secreted in extracellular vesicles (EVs), but the biological...
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Agar dehydration: a simple method for long-term storage of Acanthamoeba spp. collection at room temperature
This study describes dehydration of agar containing cysts as a novel and inexpensive method for long-term storage of Acanthamoeba spp. collections at...
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In vitro maintenance of the endosymbiont Wolbachia of Dirofilaria immitis
Wolbachia has an obligatory mutualistic relationship with many onchocercid nematodes of the subfamilies Dirofilariinae and Onchocercinae. Till date,...
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Consequence of Gamma Radiation Induced Cytomixis during Microsporogenesis in Fennel Plant (Foeniculum vulgare Mill.)
Fennel is considered as a very important spice crop with powerful therapeutic potential. An improvement in this valuable crop, selected physical...
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CRISPR/sgRNA-directed synergistic activation mediator (SAM) as a therapeutic tool for Parkinson´s disease
Parkinson`s disease (PD) is the second most prevalent neurodegenerative disease, and different gene therapy strategies have been used as experimental...
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C-type lectin receptor Dectin-1 blockade on tumour-associated macrophages improves anti-PD-1 efficacy in gastric cancer
BackgroundThis study aimed to investigate the expression and clinical significance of Dendritic cell-associated C-type lectin-1 (Dectin-1) in gastric...
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Preparation and Evaluation of Berberine-Excipient Complexes in Enhancing the Dissolution Rate of Berberine Incorporated into Pellet Formulations
Berberine is used in the treatment of metabolic syndrome and its low solubility and very poor oral bioavailability of berberine was one of the...
