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α-Synuclein Seeding Assay Using Cultured Cells
α-Synuclein, a presynaptic protein, is involved in synaptic vesicle dynamics in response to neuronal activity. Mutations of the α-synuclein gene and...
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Biochemical Assay for Measuring Diabetes Mellitus
Increased blood sugar brought on by inadequate insulin synthesis or activity is known as diabetes mellitus (DM), resulting in disrupted carbohydrate,...
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Effect of somatic antigens of Dirofilaria repens adult worms on angiogenesis, cell proliferation and migration and pseudo-capillary formation in human endothelial cells
BackgroundAngiogenesis is defined as the formation of new vessels by sprouting of endothelial cells from pre-existing vessels in response to stimuli,...
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Circular RNA circ_0058123 Targets the miR-939-5p/RAC1 Pathway to Promote the Development of Colorectal Cancer
Circular RNA (circRNA) can be used as a potential target for cancer treatment. However, the biological function and potential molecular mechanism of...
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Validation of microRNA Target Genes Using Luciferase Reporter assay and Western Blot Analysis
MicroRNA MicroRNA (miRNA) s regulate gene expressionGene expression by binding to the 3′untranslated region (UTR) of the mRNA of their target genes....
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SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway
BackgroundAs the tissue with the highest selenium content in the body, the occurrence and development of thyroid cancer are closely related to...
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Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital
BackgroundUropathogenic Escherichia coli (UPEC) is the main etiological agent behind community-acquired and hospital-acquired urinary tract...
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Cytopathic Effect (CPE )-Based Drug Screening Assay for SARS-CoV-2
Identification of an effective antiviral for the treatment of COVID-19 is considered one of the holy grails in the bid to end the pandemic. However,...
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In Vitro Screening Assay for Activators of T-Cell Migration to Solid Tumors
Recently, chimeric antigen receptor (CAR) T-cell therapy has begun to be used for solid tumors such as glioblastoma multiforme. Pediatric malignant...
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Cytochrome P450 Inhibition Assay Using Human Liver Microsomes
In vitro cytochrome P450 (CYP)-associated inhibition is the major reason for the potential clinical drug–drug interactions (DDIs). During the...
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Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology
Foodborne hepatitis A infections have been considered as a major threat for public health worldwide. Increased incidences of hepatitis A virus (HAV)...
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Serum exosomal coronin 1A and dynamin 2 as neural tube defect biomarkers
AbstractNo highly specific and sensitive biomarkers have been identified for early diagnosis of neural tube defects (NTDs). In this study, we used...
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SALL4 promotes angiogenesis in gastric cancer by regulating VEGF expression and targeting SALL4/VEGF pathway inhibits cancer progression
BackgroundSpalt-like protein 4 (SALL4) is a stemness-related transcription factor whose abnormal re-expression contributes to cancer initiation and...
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ER+ Breast Cancer Mammosphere Formation and Analysis
Mammosphere formation assays are a popular and convenient technique in the study of breast cancer by providing an in vitro mechanism by which to...
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Large-scale proximity extension assay reveals CSF midkine and DOPA decarboxylase as supportive diagnostic biomarkers for Parkinson’s disease
BackgroundThere is a need for biomarkers to support an accurate diagnosis of Parkinson’s disease (PD). Cerebrospinal fluid (CSF) has been a...
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Cytochrome P450-Mediated Drug Bioactivation Assay: An Untargeted High Resolution Accurate Mass LC/MS Assay
Glutathione trap** enables the detection and characterization of reactive metabolites which result from enzymatic bioactivation of compounds. Data...
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Molecular characterization and genetic authentication assay for Anopheles ‘hemocyte-like’ cell lines 4a-3A and 4a-3B
BackgroundAnopheles cell lines are used in a variety of ways to better understand the major vectors of malaria in sub-Saharan Africa. Despite this,...
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A 384-Well Microtiter Plate Model for Candida Biofilm Formation and Its Application to High-Throughput Screening
Candidiasis, infections caused by Candida spp., represents one of the most common nosocomial infections afflicting an expanding number of compromised...
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Circ_0000758 Facilitates Bladder Cancer Cell Growth, Migration and Angiogenesis Via Severing as miR-1236-3p Sponge
Circular RNA (circRNA) has been reported to regulate the development of bladder cancer (BCa). However, the role of circ_0000758 in BCa progression is...
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The Sister-Chromatid Exchange Assay in Human Cells
The semiconservative nature of DNA replication allows the differential labeling of sister chromatids that is the fundamental requirement to perform...