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The intracellular visualization of exogenous DNA in fluorescence microscopy
In the development of non-viral gene delivery vectors, it is essential to reliably localize and quantify transfected DNA inside the cell. To track...
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Characterization of mutations in hepatitis B virus DNA isolated from Japanese HBsAg-positive blood donors in 2021 and 2022
Missense mutations in certain small envelope proteins diminish the efficacy of antibodies. Consequently, tracking the incidence and types of...
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Identification of Valeriana fauriei and other Eurasian medicinal valerian by DNA polymorphisms in psbA–trnH intergenic spacer sequences in chloroplast DNA
In order to differentiate among Valeriana fauriei Briq. and other Eurasian medicinal valerian ( V . dioica L., V . hardwickii Wall., V . jatamansi Jones,...
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Design, Assembly, and Function of DNA Origami Mechanisms
This chapter provides an overview of the common procedures used in making functional DNA origami devices. These procedures include the design,...
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Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair
The reliance on viral vectors for the production of genetically engineered immune cells for adoptive cellular therapies remains a translational...
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Two-Dimensional DNA Origami Lattices Assembled on Lipid Bilayer Membranes
Molecular self-assembly has attracted much attention as a method to create novel supramolecular architectures. The scaffolded DNA origami method has...
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KDeep: a new memory-efficient data extraction method for accurately predicting DNA/RNA transcription factor binding sites
This paper addresses the crucial task of identifying DNA/RNA binding sites, which has implications in drug/vaccine design, protein engineering, and...
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DNA virome of ticks in the Northeast and Hubei provinces of China reveals diverse single-stranded circular DNA viruses
BackgroundTicks are medically important vectors capable of transmitting a variety of pathogens to and between host species. Although the spectrum of...
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Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Extrachromosomal DNA (ecDNA) is a common mode of oncogene amplification but is challenging to analyze. Here, we adapt CRISPR-CATCH, in vitro...
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The use of single-molecule FRET for the characterization of Holliday junctions containing human telomeric DNA: a methodological approach for nanoscale distance, mobility, and stability measurements
Single-molecule analysis has emerged as a powerful biophysical technique, allowing the investigation of molecular interactions at unprecedented...
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DNA sequence and chromatin modifiers cooperate to confer epigenetic bistability at imprinting control regions
Genomic imprinting is regulated by parental-specific DNA methylation of imprinting control regions (ICRs). Despite an identical DNA sequence, ICRs...
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Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway
Viral delivery of DNA for the targeted reprogramming of human T cells can lead to random genomic integration, and electroporation is inefficient and...
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NGS-Based Tumor-Informed Analysis of Circulating Tumor DNA
Accurate circulating tumor DNA (ctDNA) detection has an immense biomarker potential in all phases of the cancer disease course. Presence of ctDNA in...
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High-Plex ELISPOT: FOLISPOT Based on Fluorescence Detection and DNA Complementary Pairing
Enzyme-linked immunospot (ELISPOT) is one of the most important methods to measure the number of specific cells by detecting protein secretion at a...
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Distinct characteristics of the DNA damage response in mammalian oocytes
DNA damage is a critical threat that poses significant challenges to all cells. To address this issue, cells have evolved a sophisticated molecular...
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Employing toehold-mediated DNA strand displacement reactions for biomedical applications
Dynamic DNA nanotechnology belongs to a larger umbrella of DNA nanotechnology that primarily uses DNA as a nanoscopic material to build mobile...
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Extrachromosomal DNA in cancer
Extrachromosomal DNA (ecDNA) has recently been recognized as a major contributor to cancer pathogenesis that is identified in most cancer types and...
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The DNA damage sensor ATM kinase interacts with the p53 mRNA and guides the DNA damage response pathway
BackgroundThe ATM kinase constitutes a master regulatory hub of DNA damage and activates the p53 response pathway by phosphorylating the MDM2...
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Identification of Angelica acutiloba, A. sinensis, and other Chinese medicinal Apiaceae plants by DNA barcoding
Crude drug Angelicae acutilobae radix is one of the most important crude drugs in Japanese traditional medicine and is used mainly for the treatment...
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Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA
ObjectivesDevelopment of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid...