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Detection of Long Noncoding RNA Expression by Real-Time PCR
Recent advances in sequencing technology and bioinformatic analysis promote the identification of long noncoding RNA (lncRNA), a novel form of...
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The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays
BackgroundThe diagnosis of gastrointestinal nematode (GIN) infections in ruminants is routinely based on morphological/morphometric analysis of...
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Human Saliva as a Diagnostic Specimen for Early Detection of Inflammatory Biomarkers by Real-Time RT-PCR
Nowadays human saliva is more frequently studied as a non-invasive, stress-free, and preferable diagnostic material than blood. Supporting evidences...
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Phosphate-Methylated Oligonucleotides as a Novel Primer for PCR and RT-PCR
This chapter introduces neutralized DNA (nDNA) as a novel design for the primers of PCR and RT-PCR Real-time PCR (RT-PCR) by methylating phosphate...
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Development of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosis
BackgroundCanine babesiosis and anaplasmosis, caused by Babesia canis and Anaplasma phagocytophilum , respectively, are significant tick-borne...
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Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting)
Microscopic examination results in patients with filariasis are often not identified by the presence of microfilaria, so it needs to be checked by...
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Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples
The aim of this study was to identify and validate a sensitive, high-throughput, and cost-effective SARS-CoV-2 real-time RT-PCR assay to be used as a...
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Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
The pandemic coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its...
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Pooling Nasopharyngeal Swab Specimens to Improve Testing Capacity for SARS-CoV-2 by Real-Time RT-PCR
BackgroundThe detection of SARS-CoV-2 using qRT-PCR with the pooling of samples can reduce workload and costs especially when the prevalence rate of...
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Real-time PCR biochip for on-site detection of Coxiella burnetii in ticks
BackgroundQ fever, a zoonosis caused by Coxiella burnetii , has adverse effects on public health. Ticks are vectors of C. burnetii and they...
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Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity
Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection,...
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Real-time recombinase-aided amplification assay for rapid amplification of the IS1081 gene of Mycobacterium tuberculosis
Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis (TB), is the leading cause of death due to a single infectious agent...
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Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology
Foodborne hepatitis A infections have been considered as a major threat for public health worldwide. Increased incidences of hepatitis A virus (HAV)...
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Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR
BackgroundThe porcine circovirus type 2 (PCV2) is divided into eight genotypes including the previously described genotypes PCV2a to PCV2f and the...
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A multiplex real-time PCR quantitation of human herpesvirus-6, 7, 8 viruses: application in blood transfusions
BackgroundIn recent years, fluorescent quantitative polymerase chain reaction assays for detecting viral DNA are in widespread use throughout the...
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Quantitative real-time PCR in Borrelia persica tick-borne relapsing fever demonstrates correlation with the Jarisch-Herxheimer reaction
The purpose of this study is to explore whether a correlation exists between the bacterial load of Borrelia persica in tick-borne relapsing fever...
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Survey of HCMV in allogenic and autologous stem cell transplantation by real-time PCR in Kermanshah, west of Iran
IntroductionHuman Cytomegalovirus (HCMV) is the most important viral pathogen in people undergoing bone marrow transplantation (BMT). HCMV detection...
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Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method
PurposeThis study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR)...
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Real-time PCR on skin biopsies for super-spreaders’ detection in bovine besnoitiosis
BackgroundBovine besnoitiosis, an emerging disease in Europe that can be transmitted by vectors, is caused by the apicomplexan Besnoitia besnoiti ....
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Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genoty** by Fragment RT-PCR
Polymerase chain reaction (PCR) is a widely used technique in the diagnosis of viral infections due to its low cost, high sensitivity, and...