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Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
BackgroundSingle-cell RNA-seq has emerged as an innovative technology used to study complex tissues and characterize cell types, states, and lineages...
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Transcriptome Analysis Reveals Spermatogenesis-Related CircRNAs and LncRNAs in Goat Spermatozoa
Mammalian spermatozoa comprises both coding and non-coding RNAs, which are traditionally believed to be a residual of spermatogenesis. The...
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Whole exome sequencing and transcriptome analysis in two unrelated patients with novel SET mutations
The human SET nuclear proto-oncogene ( SET ) gene is a protein-coding gene that encodes proteins that affects chromatin remodeling and gene...
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Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation
BackgroundRNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of...
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Transcriptome analysis of Kunming mice responses to the bite of Xenopsylla cheopis
BackgroundFlea bites could trigger a series of complex molecular responses in the host. However, our understanding of the responses at the molecular...
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Single-Cell Sequencing in Cancer Research: Challenges and Opportunities
The single-cell field opens the door to understanding with an unprecedented level of detail how cancer arises, evolves, and responds to therapy. But...
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Whole Blood Transcriptome Analysis in Patients with Trigeminal Neuralgia: a Prospective Clinical Study
Trigeminal neuralgia (TN) brings a huge burden to patients, without long-term effective treatment. This study aimed to explore the differentially...
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Transcriptome Sequencing and Screening of Anthocyanin-Related Genes in the Leaves of Acer truncatum Bunge
Acer truncatum Bunge is generally used as an ornamental tree because of its autumn leaves, although the viewing period is short—approximately...
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Transcriptome profiling and co-expression network analysis of lncRNAs and mRNAs in colorectal cancer by RNA sequencing
BackgroundLong non-coding RNAs (lncRNAs ) are widely involved in the pathogenesis of cancers. However, biological roles of lncRNAs in occurrence and...
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Next-Generation Sequencing
The next-generation sequencing platforms can generate a large amount of high-throughput sequencing data in a short span of time. Reference-based...
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RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells
Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature....
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An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury
BackgroundThe important contribution of glia to mechanisms of injury and repair of the nervous system is increasingly recognized. In stark contrast...
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Single-cell RNA sequencing reveals cellular and molecular landscape of fetal cystic hygroma
BackgroundThe molecular mechanism of fetal cystic hygroma (CH) is still unclear, and no study has previously reported the transcriptome changes of...
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Personalized tumor combination therapy optimization using the single-cell transcriptome
BackgroundThe precise characterization of individual tumors and immune microenvironments using transcriptome sequencing has provided a great...
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Time-course transcriptome analysis of host cell response to poxvirus infection using a dual long-read sequencing approach
ObjectiveIn this study, we applied two long-read sequencing (LRS) approaches, including single-molecule real-time and nanopore-based sequencing...
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Transcriptome dataset from Solanum lycopersicum L. cv. Micro-Tom; wild type and two mutants of INDOLE-ACETIC-ACID (SlIAA9) using long-reads sequencing oxford nanopore technologies
ObjectiveTomatoes are the most widely consumed fruit vegetable and are relatively easy to cultivate. However, an increase in temperature causes some...
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Large-Scale Single-Nucleus RNA Sequencing Compatible with Complex Archived Samples
Transcriptome profiling at single-cell resolution allows us to identify and assess functional cell types and cellular states, including those within...
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Single-Cell Transcriptome Analysis of Treg
Regulatory T-cells (TregRegulatory T cell (Treg)) are considerably heterogeneous. Thymically derived TregRegulatory T cell (Treg)...
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Lymphatic metastasis-associated circRNA‒miRNA‒mRNA network for exploring the pathogenesis and therapeutic target of triple negative breast cancer based on whole-transcriptome sequencing analysis: an experimental verification study
BackgroundThe metastatic mechanisms of axillary lymph nodes (ALNs) in triple-negative breast cancer (TNBC) remain unclear. We aimed to identify the...
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Artificial image objects for classification of breast cancer biomarkers with transcriptome sequencing data and convolutional neural network algorithms
BackgroundTranscriptome sequencing has been broadly available in clinical studies. However, it remains a challenge to utilize these data effectively...
