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  1. No Access

    Article

    Heterologous expression and secretion of sweet potato peroxidase isoenzyme A1 in recombinant Saccharomyces cerevisiae

    A vector system has been developed to express isoenzyme A1 of sweet potato peroxidase (POD) and was introduced into Saccharomyces cerevisiae. The system contains the signal sequence of Aspergillus oryzae α-amylas...

    Tae-Hyeo Kim, Joon-Ki Jung, Sang-Soo Kwak, Soo-Wan Nam… in Biotechnology Letters (2002)

  2. No Access

    Article

    Optimal production of curdlan by Agrobacterium sp. with feedback inferential control of optimal pH profile

    The pH control was important for curdlan production with Agrobacterium sp. ATCC31750. Specific cell growth rate was the highest at pH 7 and the specific curdlan production rate was at pH 5.5. The pH profiles maxi...

    Jung-Heon Lee, Young Hoon Park in Biotechnology Letters (2001)

  3. No Access

    Article

    Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]

    Using lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentr...

    Vibhor Saraswat, Jeewon Lee, Dae Young Kim, Young-Hoon Park in Biotechnology Letters (2000)

  4. No Access

    Article

    Enhanced production of laccase in Trametes vesicolor by the addition of ethanol

    In a medium containing 40 g ethanol l−1, laccase production by Trametes versicolor was 2.6 unit per ml of the supernatant, which was over 20 times higher than that without ethanol. Laccase activity with ethanol w...

    In-Young Lee, Kyung-Hee Jung, Choong-Hwan Lee, Young-Hoon Park in Biotechnology Letters (1999)

  5. No Access

    Article

    Stimulation of β-carotene synthesis by hydrogen peroxide in Blakeslea trispora

    β-Carotene synthesis was increased from a negligible amount to 152 mg (g-dry cells)−1 and H2O2 was accumulated up to 16.7 μM during 2.5 day-culture of Blakeslea trispora. When cells were cultivated in 250 ml flas...

    Jae-Cheol Jeong, In-Young Lee, Seon-Won Kim, Young-Hoon Park in Biotechnology Letters (1999)

  6. No Access

    Article

    Enhancement of extracellular glucose oxidase production in pH-stat feed-back controlled fed-batch culture of recombinant Saccharomyces cerevisiae

    Glucose oxidase (GOD) from Aspergillus niger was expressed in Saccharomyces cerevisiae under the regime of GAL-10 promoter and GAL-7 terminator of S. cerevisiae and α-amylase signal sequence of Aspergillus ory...

    Arnab Kapat, Joon-Ki Jung, Young-Hoon Park in Biotechnology Letters (1998)

  7. No Access

    Article

    Enhanced production of b-carotene from Blakeslea trispora with Span 20

    Span 20 at 10 g/l induced dispersed mycelial growth of Blakeslea trispora, and significantly increased b-carotene production to 2.16 g/l. Without surfactant only 0.15 g b-carotene/l was produced. Other non-ion...

    Seon-Won Kim, Weon-Taek Seo, Young-Hoon Park in Biotechnology Letters (1997)

  8. No Access

    Article

    Increased β-carotene synthesis in Blakeslea trispora under strong alkaline culture condition

    A high yield of β-carotene, 48 mg/l, was obtained by cultivation of a strain of Blakeslea trispora under a strong alkaline culture condition (pH 10), which was significantly higher than those obtained under milde...

    Seon-Won Kim, Weon-Taek Seo, Young-Hoon Park in Biotechnology Letters (1996)

  9. No Access

    Article

    Metabolites and amino acids affecting cellular cofactor concentrations and Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus

    Additon of pyruvate or leucine was found to be efficient for increasing the intracellular ratios of NADH/NAD and NADPH/NADP while reducing the coenzyme A concentration during the cultivation of Alcaligenes eutrop...

    Mi Kyoung Kim, In Young Lee, Young Hoon Park in Biotechnology Letters (1996)

  10. No Access

    Article

    Continuous production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa

    Continuous production of D- β -hydroxyisobutyric acid (D-HIBA) from methacrylic acid (MA) using Candida rugosa IFO 0750 was most efficient under ammonium-limited conditions, whereas D-HIBA was not detected under ...

    In Young Lee, Dae Keon Choi, Chul Ho Kim, Young Hoon Park in Biotechnology Letters (1996)

  11. No Access

    Article

    Production of poly(β-hydroxybutyrate-co-β-hydroxyvalerate) from glucose and valerate in Alcaligenes eutrophus

    Production of poly(β-hydroxybulyrate-co-β-hydroxyvalcrate) [poly(HB-co-HV)] in Alcaligenes eutrophus NCIMB 11599 was investigated at various concentrations of valerate. With valerate, higher specific production r...

    In Young Lee, Mi Kyoung Kim, Guk ** Kim, Ho Nam Chang… in Biotechnology Letters (1995)

  12. No Access

    Article

    Effects of propionate on accumulation of poly(β-hydroxy-butyrate-co-β-hydroxyvalerate) and excretion of pyruvate in Alcaligenes eutrophus

    Effects of propionate on the accumulation of poly(β-hydroxybutyrate-co-β-hydroxyvalerate) and the excretion of pyruvate in Alcaligenes eutrophus were investigated at various concentrations of glucose and propiona...

    In Young Lee, Mi Kyoung Kim, Ho Nam Chang, Young Hoon Park in Biotechnology Letters (1994)

  13. No Access

    Article

    Ethyl acetate production in the gas phase

    Ethyl acetate was produced from gaseous ethanol and acetic acid using a lipase from Porcine Pancreas at 25 °C. Its production was investigated at different moisture content of lipase enzyme powder because it i...

    Soon Ook Hwang, Young Hoon Park in Biotechnology Letters (1994)