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  1. Article

    Open Access

    Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner

    Despite of the fact that mammalian genomes are far more spacious than prokaryotic genomes, recent nucleotide sequencing data have revealed that many mammalian genes are arranged in a head-to-head orientation a...

    **-Yen Chen, Wun-Shaing W Chang, Ruey-Hwang Chou, Yiu-Kay Lai… in BMC Molecular Biology (2007)

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  2. No Access

    Article

    Stable RNA Interference in Spodoptera frugiperda Cells by a DNA Vector-based Method

    Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cell...

    Chih-Chien Lin, John T. -A. Hsu, Kai-Ling Huang, Hung-Kuan Tang… in Biotechnology Letters (2006)

  3. Article

    Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

    Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatogra...

    Chia-Wei Lai, Yao-Chi Chung, Yiu-Kay Lai, Margaret Dah-Tsyr Chang… in Biotechnology Letters (2005)

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  4. No Access

    Article

    Autophosphorylation-dependent protein kinase phosphorylates Ser25, Ser38, Ser65, Ser71, and Ser411 in vimentin and thereby inhibits cytoskeletal intermediate filament assembly

    The autophosphorylation-dependent protein kinase has been identified as a potent vimentin kinase that incorporates 2 mol of phosphates per mol of protein and generates five major phosphorylation sites in vimen...

    Tze-Jen Huang, Tsong-Tse Lee, Wen-Chuan Lee, Yiu-Kay Lai… in Journal of Protein Chemistry (1994)

  5. No Access

    Article

    A maize cryptic Ac-homologous sequence derived from an Activator transposable element does not transpose

    Sequences sharing homology to the transposable element Activator (Ac) are prevalent in the maize genome. A cryptic Ac-like DNA, cAc-11, was isolated from the maize inbred line 4Co63 and sequenced. Cryptic Ac-11 h...

    Jun-Yi Len, Y. Henry Sun, Yiu-Kay Lai, Jychian Chen in Molecular and General Genetics MGG (1992)

  6. No Access

    Article

    Physical maps of Nicotiana chloroplast DNA constructed by an efficient procedure

    The restriction profiles of chloroplast DNA (cpDNA) from Nicotiana tabacum, N. sylvestris, N. plumbaginifolia, and N. otophora were obtained with respect to AvaI, BamHI, BglI, HindIII, PstI, PvuII, SalI, and XhoI...

    Ding-I Yang, Teng-Yung Feng, Chi-Chang Chen… in Theoretical and Applied Genetics (1992)