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  1. No Access

    Article

    Construction, Expression and Refolding of a Bifunctional Fusion Protein Consisting of C-Terminal 12-Residue of Hirudin-PA and Reteplase

    To obtain a bifunctional protein simultaneously showing bioactivity of anticoagulant and fibrinolytic for use in the treatment of thrombotic diseases, we constructed a fusion protein (HV12p–rPA) containing C-t...

    Ling Gao, Chun Zhang, Lingling Li, Lan Liang, Xuan Deng, Wutong Wu… in The Protein Journal (2012)

  2. No Access

    Article

    Cytoglobin Exhibits Anti-Fibrosis Activity on Liver In Vivo and In Vitro

    Cytoglobin, generated using genetic engineering method, is a kind of recombinant human stellate cell activation-associated protein. We speculate that it could influence the development of hepatic fibrosis like...

    **n He, Ruoyun Lv, Ke Wang, **aofeng Huang, Wutong Wu, Lifang Yin… in The Protein Journal (2011)

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    Article

    Coupled Chromatography for Assay of the Venom Proteome of the Snake Agkistrodon acutus: An Effective Strategy for Discovery of Active Components

    Two-dimensional liquid chromatography (2D-LC) is an attractive option for proteome profiling because of its advantages in separation and analysis of proteins and peptides of extreme pH and molecular weight. Pr...

    Chunhong Huang, **aodong Huang, Yi Kong, Wutong Wu in Chromatographia (2009)

  4. No Access

    Article

    Enhanced secretion of adhesive recognition sequence containing hirudin III mutein in E. coli

    It has been previously shown that Escherichia coli l-asparaginase II (l-ASP) signal peptide is capable of being utilized to direct extracellular secretion of hirudin III (HV3) in shake fla...

    Shuhua Tan, Wutong Wu, **angyu Li, Li Cui, Bing Li, Qi** Ruan in Molecular Biotechnology (2007)