11 Result(s)
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Article
Open AccessGenome-wide distribution of 5-hydroxymethyluracil and chromatin accessibility in the Breviolum minutum genome
In dinoflagellates, a unique and extremely divergent genomic and nuclear organization has evolved. The highly unusual features of dinoflagellate nuclei and genomes include permanently condensed liquid crystall...
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Open AccessThe chromatin landscape of the euryarchaeon Haloferax volcanii
Archaea, together with Bacteria, represent the two main divisions of life on Earth, with many of the defining characteristics of the more complex eukaryotes tracing their origin to evolutionary innovations fir...
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Article
Open AccessCasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA map**
Detecting and mitigating off-target activity is critical to the practical application of CRISPR-mediated genome and epigenome editing. While numerous methods have been developed to map Cas9 binding specificity...
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Open AccessTargeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Extrachromosomal DNA (ecDNA) is a common mode of oncogene amplification but is challenging to analyze. Here, we adapt CRISPR-CATCH, in vitro CRISPR-Cas9 treatment and pulsed field gel electrophoresis of agaros...
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Open AccessSingle-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer
To chart cell composition and cell state changes that occur during the transformation of healthy colon to precancerous adenomas to colorectal cancer (CRC), we generated single-cell chromatin accessibility prof...
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Open AccessAuthor Correction: ArchR is a scalable software package for integrative single-cell chromatin accessibility analysis
A Correction to this paper has been published: https://doi.org/10.1038/s41588-021-00850-x.
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Article
Open AccessTranscription-dependent domain-scale three-dimensional genome organization in the dinoflagellate Breviolum minutum
Dinoflagellate chromosomes represent a unique evolutionary experiment, as they exist in a permanently condensed, liquid crystalline state; are not packaged by histones; and contain genes organized into tandem ...
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Open AccessArchR is a scalable software package for integrative single-cell chromatin accessibility analysis
The advent of single-cell chromatin accessibility profiling has accelerated the ability to map gene regulatory landscapes but has outpaced the development of scalable software to rapidly extract biological mea...
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Correction: Corrigendum: Landscape of monoallelic DNA accessibility in mouse embryonic stem cells and neural progenitor cells
Nat. Genet.; 10.1038/ng.3769; corrected online 13 February 2017 In the version of this article initially published online, there were two errors. In the section “Three classes of monoallelic elements” in the m...
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Open AccessSingle-cell epigenomic variability reveals functional cancer heterogeneity
Cell-to-cell heterogeneity is a major driver of cancer evolution, progression, and emergence of drug resistance. Epigenomic variation at the single-cell level can rapidly create cancer heterogeneity but is dif...
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Open AccessThe future of sequencing: convergence of intelligent design and market Darwinism
A report on the Advances in Genome Biology and Technology meeting held in Marco Island, Florida, USA, on February 12–15, 2014.
