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Article
Transfection of heteroduplexes containing uracil · guanine or thymine · guanine mispairs into plant cells
We have compared the fate of U · G mispairs or analogous T · G mispairs in DNA heteroduplexes transfected into tobacco protoplasts. The heteroduplex DNA consisted of tomato golden mosaic virus DNA sequences in...
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Article
DNA methylation inhibits propagation of tomato golden mosaic virus DNA in transfected protoplasts
The effects of methylation on plant viral DNA replication have been studied inNicotiana tabacum protoplasts transfected with DNA of the geminivirus tomato golden mosaic virus (TGMV). The transfected cells were al...
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Article
Agrobacterium-mediated inoculation of plants with tomato golden mosaic virus DNAs
We have adapted the “agroinfection” procedure of Grimsley and co-workers [4,5] to develop a simple, efficient, reproducible infectivity assay for the insect-transmitted, split-genome geminivirus, tomato golden...
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Article
Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants
Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, althou...
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Article
The influence of whole lake aeration on the limnology of a hypereutrophic lake in central Florida
To determine the influence of a multiple inversion aeration system upon the limnology of a small sinkhole lake, we monitored physical-chemical and biological parameters for 15 months prior to starting aeration...
