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    Article

    Differentiation of flue-cured tobacco leaves in different positions based on neutral volatiles with principal component analysis (PCA)

    Differentiation of tobacco leaves in different plant stalk positions in China was achieved based on basic chemical compounds and neutral volatiles by principal component analysis (PCA). The continuous flow ana...

    Weifeng Sun, Zhilei Zhou, Yue Li, Zhiqiang Xu in European Food Research and Technology (2012)

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    Article

    Influence of various factors on formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) in a solid-state model system of Maillard reaction

    The influences of various factors on the formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) were investigated in a solid-state Maillard reaction system. Thermogravimetric analysis (TG) and di...

    Zhilei Zhou, Zhiqiang Xu, Junsheng Shu, Shike She in European Food Research and Technology (2014)

  3. Article

    Open Access

    Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

    The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from

    Weifeng Sun, Longbao Zhu, **anggui Chen, ** Chen, Lingling Yang in BMC Biotechnology (2016)

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    Article

    The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swap** the C-terminal Domain of β subunit

    The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit ...

    Weifeng Sun, Longbao Zhu, **anggui Chen in Applied Biochemistry and Biotechnology (2016)

  5. No Access

    Article

    Activity Enhancement Based on the Chemical Equilibrium of Multiple-Subunit Nitrile Hydratase from Bordetella petrii

    The maturation mechanism of nitrile hydratase (NHase) of Pseudomonas putida NRRL-18668 was discovered and named as “self-subunit swap**.” Since the NHase of Bordetella petrii DSM 12804 is similar to that of P. ...

    Yi Liu, ** Liu, Lu Lin, Yueqin Zhao in Applied Biochemistry and Biotechnology (2016)

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    Article

    An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis

    Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (...

    Weifeng Sun, Yuanming Wu, Wenwu Ding, Li Wang in Bioprocess and Biosystems Engineering (2020)

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    Article

    Location and map** of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequencing

    Northern corn leaf blight (NCLB) is one of the main diseases of maize, which greatly reduces production and causes millions of dollars in losses worldwide annually. Identification of NCLB resistance genes in m...

    Chunlei Li, Fenglou Ling, Guihua Su, Weifeng Sun, Hongshuang Liu in Molecular Breeding (2020)