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Article
Differentiation of flue-cured tobacco leaves in different positions based on neutral volatiles with principal component analysis (PCA)
Differentiation of tobacco leaves in different plant stalk positions in China was achieved based on basic chemical compounds and neutral volatiles by principal component analysis (PCA). The continuous flow ana...
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Article
Influence of various factors on formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) in a solid-state model system of Maillard reaction
The influences of various factors on the formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) were investigated in a solid-state Maillard reaction system. Thermogravimetric analysis (TG) and di...
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Article
Open AccessSuccessful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115
The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from
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Article
The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swap** the C-terminal Domain of β subunit
The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit ...
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Article
Activity Enhancement Based on the Chemical Equilibrium of Multiple-Subunit Nitrile Hydratase from Bordetella petrii
The maturation mechanism of nitrile hydratase (NHase) of Pseudomonas putida NRRL-18668 was discovered and named as “self-subunit swap**.” Since the NHase of Bordetella petrii DSM 12804 is similar to that of P. ...
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Article
An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis
Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (...
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Article
Location and map** of the NCLB resistance genes in maize by bulked segregant analysis (BSA) using whole genome re-sequencing
Northern corn leaf blight (NCLB) is one of the main diseases of maize, which greatly reduces production and causes millions of dollars in losses worldwide annually. Identification of NCLB resistance genes in m...