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Article
An auto-inducible expression and high cell density fermentation of Beefy Meaty Peptide with Bacillus subtilis
Currently, some cases about the expression of flavor peptides with microorganisms were reported owing to the obvious advantages of biological expression over traditional methods. However, beefy meaty peptide (...
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Article
Activity Enhancement Based on the Chemical Equilibrium of Multiple-Subunit Nitrile Hydratase from Bordetella petrii
The maturation mechanism of nitrile hydratase (NHase) of Pseudomonas putida NRRL-18668 was discovered and named as “self-subunit swap**.” Since the NHase of Bordetella petrii DSM 12804 is similar to that of P. ...
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Article
The Stability Enhancement of Nitrile Hydratase from Bordetella petrii by Swap** the C-terminal Domain of β subunit
The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit ...
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Article
Open AccessSuccessful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115
The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from
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Article
Effect of exogenous softwood on thermal decomposition of reconstituted tobacco sheet
In recent years, reconstituted tobacco sheet (RTS) has played an increasingly vital role in reducing tumorigenicity for tobacco industry. In this paper, two plant fibers of tobacco pulp (TP) and softwood pulp ...
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Article
Influence of various factors on formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) in a solid-state model system of Maillard reaction
The influences of various factors on the formation of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) were investigated in a solid-state Maillard reaction system. Thermogravimetric analysis (TG) and di...
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Article
Study on the effect of potassium lactate additive on the combustion behavior and mainstream smoke of cigarettes
The influence of potassium lactate (PL) on the combustion behavior and semi-volatile compounds of tobacco during smoking is investigated in this study. The addition of PL showed no effect on the content of tot...
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Article
The generation of carbon monoxide and carbonyl compounds in reconstituted tobacco sheet
In recent years, reconstituted tobacco sheet (RTs) has played an increasingly significant role in tobacco industry. The yields of CO and carbonyl compounds (formaldehyde, acetaldehyde, acetone, acrolein, propa...
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Article
Differentiation of flue-cured tobacco leaves in different positions based on neutral volatiles with principal component analysis (PCA)
Differentiation of tobacco leaves in different plant stalk positions in China was achieved based on basic chemical compounds and neutral volatiles by principal component analysis (PCA). The continuous flow ana...