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    Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

    Flavin-based fluorescent proteins (FbFPs) are small fluorescent proteins derived from light-oxygen-voltage (LOV) domains. The proteins bind ubiquitous endogenous flavins as chromophores and can be used as vers...

    Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov… in Fluorescent Proteins (2023)

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    Article

    Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species

    Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind diffe...

    Anastasia Smolentseva, Ivan M. Goncharov… in Photochemical & Photobiological Sciences (2021)

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    Article

    A thermostable flavin-based fluorescent protein from Chloroflexus aggregans: a framework for ultra-high resolution structural studies

    Light-Oxygen-Voltage (LOV) domains are conserved parts of photoreceptors in plants, bacteria and fungi that bind flavins as chromophores and detect blue light. In the past, LOV domain variants have been develo...

    Vera V. Nazarenko, Alina Remeeva, Anna Yudenko… in Photochemical & Photobiological Sciences (2019)