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within Takeshi Fukuda Remove this filter Life Sciences Remove this filter Microbial Genetics and Genomics Remove this filter Microbiology Remove this filter

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  1. No Access

    Article

    Demonstration of catalytic proton acceptor of chitosanase from Paenibacillus fukuinensis by comprehensive analysis of mutant library

    Chitosanase from Paenibacillus fukuinensis D2 is an attractive enzyme, and it exhibits both chitosanase and β-1, 4 glucanase activities. In our previous study, we generated P. fukuinensis chitosanase-displaying y...

    Danya Isogawa, Takeshi Fukuda, Kouichi Kuroda… in Applied Microbiology and Biotechnology (2009)

  2. No Access

    Article

    Improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain

    To utilize glucoamylase-displaying yeast cells for enzymatic desizing of starched cotton cloth, we constructed yeast strains that codisplayed Rhizopus oryzae glucoamylase and two kinds of Trichoderma reesei cellu...

    Takeshi Fukuda, Michiko Kato-Murai… in Applied Microbiology and Biotechnology (2008)

  3. No Access

    Article

    Enhancement of substrate recognition ability by combinatorial mutation of β-glucosidase displayed on the yeast cell surface

    Recently, in family 3 β-glucosidase (BGL), the catalytically important Asp nucleophile has been identified in the SDW segment of the SDWG sequence by site-directed mutagenesis. However, the details about the r...

    Takeshi Fukuda, Michiko Kato-Murai… in Applied Microbiology and Biotechnology (2007)