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Article
Isolation and characterization of the nuclease O gene (nucO) from Aspergillus oryzae
Nuclease O in the mycelia of Aspergillus oryzae has been purified 55-fold by successive steps of chromatography from the filtrate of the autolyzate. The molecular mass of nuclease O was 32 kDa, as estimated by S...
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Article
Transfer of an insecticidal protein gene ofBacillus thuringiensis into plant-colonizingAzospirillum
A fusion plasmid, pRKC, was constructed, using pACYC184, RSF1010 and a kanamycin-resistance cartridge from pUC4K, to convey thecryIA(a) gene intoAzospirillum spp. With the pRKC plasmid, the number of putative tra...
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Chapter and Conference Paper
Construction and Characterization of Chimeric Proteins Between Pyocins and Colicin E3
Pyocins S1, S2 and AP41 are the most frequently found protease-sensitive bacteriocins inPseudomonas aeruginosa. They are classified by the different receptor specificities. A peculiar feature of these bacteriocin...
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Chapter and Conference Paper
Immunity Specificity and Evolution of the Nuclease-Type E Colicins
E-Group colicins are encoded by respective ColE plasmids and kill Escherichia coli cells through binding to a common surface receptor, BtuB, which is also the receptor for phage BF23 and vitamin B12 uptake (Buxto...