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Article
Analysis of the barley chromosome 2 region containing the six-rowed spike gene vrs1 reveals a breakdown of rice–barley micro collinearity by a transposition
In cultivated barley (Hordeum vulgare ssp. vulgare), six-rowed spikes produce three times as many seeds per spike as do two-rowed spikes. The determinant of this trait is the Mendelian gen...
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Article
Inter-subspecific maps of non-brittle rachis genes btr1/btr2 using occidental, oriental and wild barley lines
Brittle rachis of wild barley is controlled by two dominant complementary genes, Btr1 and Btr2, and mutation in either locus (btr1 or btr2) results in the non-brittle rachis of cultivated barley. In this study, a...
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Article
Structural and distributional variation of mitochondrial rps2 genes in the tribe Triticeae (Poaceae)
The mitochondrial rps2 gene from barley, like that of rice, wheat, and maize, has an extended open reading frame (ORF) at the 3′-region when compared to that from lower plants. However, the extended portions are ...
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Article
High-density AFLP map of nonbrittle rachis 1 (btr1) and 2 (btr2) genes in barley (Hordeum vulgare L.)
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Article
High-density AFLP map of nonbrittle rachis 1 (btr1) and 2 (btr2) genes in barley (Hordeum vulgare L.)
Wild relatives of barley disperse their seeds at maturity by means of their brittle rachis. In cultivated barley, brittleness of the rachis was lost during domestication. Nonbrittle rachis of occidental barley...
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Article
Identification and map** of cleistogamy genes in barley
Cleistogamy is a closed type of flowering with ensured self-pollination and an important trait to study evolutionary development in flower organs, reproduction systems, gene flow, and disease control. Still, v...
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Article
Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.)
Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was develo...
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Article
Molecular map** of the intermedium spike-c (int-c) and non-brittle rachis 1 (btr1) loci in barley (Hordeum vulgare L.)
Lateral spikelet fertility and a non-brittle rachis are key characters in studying the evolution of barley. The fertility of lateral spikelets is controlled predominantly by the alleles at the vrs1 locus on chrom...
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Article
A DNA marker closely linked to the vrs1 locus (row-type gene) indicates multiple origins of six-rowed cultivated barley (Hordeum vulgare L.)
The origin of six-rowed cultivated barley was studied using a DNA marker cMWG699 closely linked to the vrs1 locus. Restriction patterns of the PCR-amplified product of the cMWG699 locus were examined in 280 culti...
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Article
Molecular map** of a fertility restoration locus (Rfm1) for cytoplasmic male sterility in barley (Hordeum vulgare L.)
The Rfm1a gene restores the fertility of msm1 cytoplasmic male-sterile lines in barley. We identified three RAPD markers linked to the Rfm1 locus (CMNB-07/800, OPI-18/900, and OPT-02/700) using isogenic lines and...
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Article
Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.)
In order to identify sequence-tagged sites (STSs) appropriate for recombinant inbred lines (RILs) of barley cultivars ‘Azumamugi’ × ‘Kanto Nakate Gold’, a total of 43 STS primer pairs were generated on the ba...
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Article
Map** quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L.
Quantitative trait loci (QTL) controlling the regeneration ability of rice seed callus were detected using 245 RFLP markers and 98 BC1F5 lines derived from two varieties, ‘Nipponbare’ and ‘Kasalath’. Regeneratio...
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Article
Identification of random amplified polymorphic DNA (RAPD) markers linked to the v locus in barley, Hordeum vulgare L.
Recombinant backcross lines of barley were produced from a cross between Kanto Nakate Gold (KNG; two-rowed) and Azumamugi (AZ; six-rowed) after backcrosses of F1 plants with AZ as the recurrent parent. Each of t...
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Article
Comparison of two indices for evaluating regeneration ability in rice (Oryza sativa L.) through a diallel analysis
A full diallel analysis was performed among seven rice cultivars, all of which showed different abilities of regeneration from seed-derived calli. Number of regenerated shoots and regeneration frequency were ...
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Article
Genetic map** of a quantitative trait locus (QTL) that enhances the shoot differentiation rate in Hordeum vulgare L.
A quantitative trait locus (QTL) controlling shoot differentiation from immature embryo callus was identified by linkage analysis with morphological and isozyme markers in barley, Hordeum vulgare L. Immature embr...
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Article
Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max
Soybean somatic embryos were induced from cultured immature embryos in the presence of a highlevel concentration of Naphthalenacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenetic capacity...