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Article
Production of phytoestrogen S-equol from daidzein in mixed culture of two anaerobic bacteria
An anaerobic incubation mixture of two bacterial strains Eggerthella sp. Julong 732 and Lactobacillus sp. Niu-O16, which have been known to transform dihydrodaidzein to S-equol and daidzein to dihydrodaidzein res...
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Article
Cis-2′, 3′-Dihydrodiol production on flavone B-Ring by Biphenyl Dioxygenase from Pseudomonas pseudoalcaligenes KF707 expressed in Escherichia coli
Escherichia coli JM109 strains expressing either toluene dioxygenase from Pseudomonas putida F1 or biphenyl dioxygenase from Pseudomonas pseudoalcaligenes KF707 were examined for their ability to...
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Article
Normalization of reverse transcription quantitative-PCR with housekee** genes in rice
Reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR) is useful for the systematic measurement of plant physiological changes in gene expression. The validity of using 1...
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Article
Design of an expression vector and its application to heterologous protein expression in Bacillus subtilis
An expression vector, pUBEX, was constructed for extracellular production of heterologous proteins in Bacillus subtilis using a polyhistidine tag on the C-terminal sequence, providing an efficient and easy purifi...
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Article
Molecular cloning and sequence analysis of an endo-inulinase gene from Arthrobacter sp.
A 3.0-kb DNA fragment containing an endo-inulinase gene was cloned from Arthrobacter sp. S37. It contained a single open reading frame of 2439 bp, encoding a polypeptide composed of signal peptide of 53 amino ...