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Article
3D super-localization of intracellular organelle contacts at live single cell by dual-wavelength synchronized fluorescence-free imaging
A fluorescence-free real-time three-dimensional (3D) super-localization method for the analysis of 3D structure of organelles (e.g., mitochondria-associated endoplasm reticulum [mito-ER] contacts) in live sing...
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Protocol
Fast High-Throughput Screening of H1N1 Virus by Parallel Detection with Multichannel Microchip Electrophoresis
Influenza is one of the acute respiratory diseases of human caused by the influenza A (H1N1) virus and accounted for major public health concerns worldwide. The polymerase chain reaction (PCR) methods are the ...
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Article
Quantification of UV-induced cyclobutane pyrimidine dimers using an oligonucleotide chip assay
A lesion-specific enzyme-induced DNA strand break assay was developed for an oligonucleotide chip for the determination of UVB-induced cyclobutane pyrimidine dimers (CPDs). A 20-mer of fluorophore-labeled and ...
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Article
Detection of single-molecule DNA hybridization by using dual-color total internal reflection fluorescence microscopy
We examined the use of prism-type simultaneous dual-color total internal reflection fluorescence microscopy (TIRFM) to probe DNA molecules at the single-molecule level. The system allowed the direct detection ...