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Open AccessReal-time six-dimensional spatiotemporal tracking of single anisotropic nanoparticles in live cells by integrated multifunctional light-sheet nanoscopy
An integrated multifunctional light-sheet nanoscopy (iMLSN) combined with differential interference contrast, total internal reflection, epifluorescence, a super-resolution radial fluctuation-stream module, and a...
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Article
Open AccessQuantifying intracellular trafficking of silica-coated magnetic nanoparticles in live single cells by site-specific direct stochastic optical reconstruction microscopy
Nanoparticles have been used for biomedical applications, including drug delivery, diagnosis, and imaging based on their unique properties derived from small size and large surface-to-volume ratio. However, co...
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Article
Open AccessDecrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles
Nanoparticles are being increasingly used in biomedical applications owing to their unique physical and chemical properties and small size. However, their biophysical assessment and evaluation of side-effects ...
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Article
3D super-localization of intracellular organelle contacts at live single cell by dual-wavelength synchronized fluorescence-free imaging
A fluorescence-free real-time three-dimensional (3D) super-localization method for the analysis of 3D structure of organelles (e.g., mitochondria-associated endoplasm reticulum [mito-ER] contacts) in live sing...
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Article
Quantification of UV-induced cyclobutane pyrimidine dimers using an oligonucleotide chip assay
A lesion-specific enzyme-induced DNA strand break assay was developed for an oligonucleotide chip for the determination of UVB-induced cyclobutane pyrimidine dimers (CPDs). A 20-mer of fluorophore-labeled and ...
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Article
Detection of single-molecule DNA hybridization by using dual-color total internal reflection fluorescence microscopy
We examined the use of prism-type simultaneous dual-color total internal reflection fluorescence microscopy (TIRFM) to probe DNA molecules at the single-molecule level. The system allowed the direct detection ...