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Article
Structural characterization of full-length NSF and 20S particles
The 20S particle, which is composed of NSF, SNAP and the SNARE complex, is important in fusion events. Single-particle cryo-EM and negative stain EM studies of the 20S particle and of NSF in its different nucl...
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Article
Lipid rafts association of synaptotagmin I on synaptic vesicles
We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca2+ was found to positively re...
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Article
Overexpression of complexin in PC12 cells inhibits exocytosis by preventing SNARE complex recycling
Complexin is an important protein that functions during Ca2+-dependent neurotransmitter release. Substantial evidence supports that complexin performs its role through rapid interaction with SNARE complex with hi...
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Article
Change in pH-dependent Membrane Insertion Characteristics of Trichosanthin Caused by Deletion of Its Last Seven C-terminal Amino Acid Residues
Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) that can selectively kill some types of cells at low concentration (0.1-1 nM). The pH-dependent membrane insertion ability of TCS makes it po...
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Chapter
Study of the relationship between structure and function of HIV-1 gp 41 N terminus fusion peptide
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Chapter
Conformational changes of bovine pancreatic polypeptide (BPP) as it interacts with lipids
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Article
Numerical simulations of surface plasmon resonance system for monitoring DNA hybridization and detecting protein-lipid film interactions
This paper presents a simple method to extract information about thin organic films from surface plasmon resonance (SPR) spectra. From numerical simulations it was found that a shift (Δθ SPR) of an absorption pe...
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Article
Specific binding of avidin to biotin containing lipid lamella surfaces studied with monolayers and liposomes
The interaction of avidin (from egg white) with phospholipid (monolayer and bilayer) model membranes containing biotin-conjugated phospholipids has been studied. In the first part, using surface sensitive tech...
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Article
Induction of changes in the secondary structure of globular proteins by a hydrophobic surface
Circular dichroism, ellipsometry and radiolabeling techniques were employed to study the induction of changes in the secondary structure of BSA, myoglobin and cytochrome C by a hydrophobic surface. The results...