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Open AccessThe structural basis for light acclimation in phycobilisome light harvesting systems systems in Porphyridium purpureum
Photosynthetic organisms adapt to changing light conditions by manipulating their light harvesting complexes. Biophysical, biochemical, physiological and genetic aspects of these processes are studied extensiv...
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Article
Open AccessNear-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex
Nuclear pore complexes (NPCs) mediate bidirectional nucleocytoplasmic transport of substances in eukaryotic cells. However, the accurate molecular arrangement of NPCs remains enigmatic owing to their huge size...
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Article
Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063
Photosystem II (PSII) is a multisubunit pigment–protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analy...
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Open AccessAntenna arrangement and energy-transfer pathways of PSI–LHCI from the moss Physcomitrella patens
Plants harvest light energy utilized for photosynthesis by light-harvesting complex I and II (LHCI and LHCII) surrounding photosystem I and II (PSI and PSII), respectively. During the evolution of green plants...
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Article
Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits
Photosystem I (PSI) is a highly efficient natural light-energy converter, and has diverse light-harvesting antennas associated with its core in different photosynthetic organisms. In green algae, an extremely ...
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Article
Open AccessStructural basis for specific flagellin recognition by the NLR protein NAIP5
The nucleotide-binding domain- and leucine-rich repeat (LRR)-containing proteins (NLRs) function as intracellular immune receptors to detect the presence of pathogen- or host-derived signals. The mechanisms of...
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Article
Cryo-EM structure of Nma111p, a unique HtrA protease composed of two protease domains and four PDZ domains
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Article
Structural organization of an intact phycobilisome and its association with photosystem II
Phycobilisomes (PBSs) are light-harvesting antennae that transfer energy to photosynthetic reaction centers in cyanobacteria and red algae. PBSs are supermolecular complexes composed of phycobiliproteins (PBPs...
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Article
Cryo-EM structure of SNAP-SNARE assembly in 20S particle
N-ethylmaleimide-sensitive factor (NSF) and α soluble NSF attachment proteins (α-SNAPs) work together within a 20S particle to disassemble and recycle the SNAP receptor (SNARE) complex after intracellular memb...
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Article
Structural characterization of full-length NSF and 20S particles
The 20S particle, which is composed of NSF, SNAP and the SNARE complex, is important in fusion events. Single-particle cryo-EM and negative stain EM studies of the 20S particle and of NSF in its different nucl...
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Article
Trichosanthin inhibits integration of human immunodeficiency virus type 1 through depurinating the long-terminal repeats
Trichosanthin (TCS) is a type I ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus type 1. However, the anti-viral mechanism remains elusive. By a well-accepted ...
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Article
Lipid rafts association of synaptotagmin I on synaptic vesicles
We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca2+ was found to positively re...
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Article
Overexpression of complexin in PC12 cells inhibits exocytosis by preventing SNARE complex recycling
Complexin is an important protein that functions during Ca2+-dependent neurotransmitter release. Substantial evidence supports that complexin performs its role through rapid interaction with SNARE complex with hi...
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Article
The identification of a new actin-binding region in p57
The actin-binding protein p57 is a member of mammalian coronin-like proteins. The roles of this protein in phagocytic processes conceivably depend on its interactions with F-actin. Two regions, p571-34 and p57111...
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Article
Change in pH-dependent Membrane Insertion Characteristics of Trichosanthin Caused by Deletion of Its Last Seven C-terminal Amino Acid Residues
Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) that can selectively kill some types of cells at low concentration (0.1-1 nM). The pH-dependent membrane insertion ability of TCS makes it po...
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Study of the relationship between structure and function of HIV-1 gp 41 N terminus fusion peptide
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Conformational changes of bovine pancreatic polypeptide (BPP) as it interacts with lipids
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Numerical simulations of surface plasmon resonance system for monitoring DNA hybridization and detecting protein-lipid film interactions
This paper presents a simple method to extract information about thin organic films from surface plasmon resonance (SPR) spectra. From numerical simulations it was found that a shift (Δθ SPR) of an absorption pe...
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Specific binding of avidin to biotin containing lipid lamella surfaces studied with monolayers and liposomes
The interaction of avidin (from egg white) with phospholipid (monolayer and bilayer) model membranes containing biotin-conjugated phospholipids has been studied. In the first part, using surface sensitive tech...