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Article
Complement test for distinctiveness, uniformity and stability testing of kharif onion (Allium cepa L.) varieties
The distinctiveness, uniformity and stability (DUS) testing is important for varietal identification and protection of plant variety. It has become one of the important assays in scientific studies and consequ...
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Article
First insights into the biochemical and molecular response to cold stress in Cicer microphyllum, a crop wild relative of chickpea (Cicer arietinum)
Identifying a potential crop wild relative (CWR) of legumes, especially one with high abiotic stress tolerance, has been a priority of plant breeders for many decades. Traditionally CWRs have been selected bas...
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Article
Isolation and characterization of cold inducible genes in carrot by suppression subtractive hybridization
Daucus carota is cultivated widely but grows best in cool climates. Suppression subtractive hybridization (SSH) is a PCR based method used to selectively amplify differentially expressed cDNAs an...
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Article
Marker-free transgenic cucumber expressing Arabidopsis cbf1 gene confers chilling stress tolerance
Marker-free transgenic cucumber (Cucumis sativus L.) cv. Poinsett 76 SR plants were produced by Agrobacterium mediated transformation. A transformation efficiency of 1.62 was observed on using Agrobacterium tumef...
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Article
The cowpea trypsin inhibitor promoter drives expression in response to cellular maturation in Arabidopsis thaliana
The bowman-birk type trypsin inhibitors accumulate in high concentration in legume and cereal seeds, especially during seed maturation and are considered to be involved in insect tolerance. The 5′ flanking seq...
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Article
Agrobacterium mediated genetic transformation of summer squash (Cucurbita pepo L. cv. Australian green) with cbf-1 using a two vector system
Efficient plant regeneration via shoot tip provided a basis for the optimization of the genetic transformation protocol. Therefore, experiments were conducted to establish an efficient in vitro regeneration pr...