8 Result(s)
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Article
Open AccessDandelion uses the single-cell adaptive immune receptor repertoire to explore lymphocyte developmental origins
Assessment of single-cell gene expression (single-cell RNA sequencing) and adaptive immune receptor (AIR) sequencing (scVDJ-seq) has been invaluable in studying lymphocyte biology. Here we introduce Dandelion,...
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Article
Open AccessDMRT1 regulates human germline commitment
Germline commitment following primordial germ cell (PGC) specification during early human development establishes an epigenetic programme and competence for gametogenesis. Here we follow the progression of nas...
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Article
Cell2location maps fine-grained cell types in spatial transcriptomics
Spatial transcriptomic technologies promise to resolve cellular wiring diagrams of tissues in health and disease, but comprehensive map** of cell types in situ remains a challenge. Here we present сell2locat...
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Protocol
Inference of Ligand–Receptor Pairs from Single-Cell Transcriptomics Data
Cell–cell communication is crucial for development and tissue homeostasis in multicellular organisms. Single-cell transcriptomics has emerged as a revolutionary technique for dissecting cellular compositions a...
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Article
CellPhoneDB: inferring cell–cell communication from combined expression of multi-subunit ligand–receptor complexes
Cell–cell communication mediated by ligand–receptor complexes is critical to coordinating diverse biological processes, such as development, differentiation and inflammation. To investigate how the context-dep...
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Article
Exponential scaling of single-cell RNA-seq in the past decade
Here Sarah Teichmann and colleagues provide a Perspective on the exponential scaling of single-cell RNA-sequencing experiments over the last decade, commenting on the methodological developments that have unde...
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Article
Open AccessIL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation
The role of cytokines in establishing specific transcriptional programmes in innate immune cells has long been recognized. However, little is known about how these extracellular factors instruct innate immune ...
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Article
Open AccessNF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation
Monocyte-to-osteoclast conversion is a unique terminal differentiation process that is exacerbated in rheumatoid arthritis and bone metastasis. The mechanisms implicated in upregulating osteoclast-specific gen...
