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    An improved integrative transformation system for Pichia pastoris with DNA-polyethylenimine-dextran sulfate nanoparticles

    Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it ...

    Wei Xue, Hang Wang, Zuodi Pei, Wei Zhao… in Biotechnology and Bioprocess Engineering (2013)