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Article
High expression of EPO gene using un-dhfr negative cell
Erythropoietin (EPO) genomic gene was cloned and its expression vector pOP13/EPO was constructed. CHO-K12 cell was transfected by this vector using lipofectin method. A stable expression cell strain C10 cell w...
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Article
Effects of clinorotation on COL1A1-EGFP gene expression
Bone-formation related gene plays a critical role in bone loss induced by space microgravity, however the exact mechanism is unclear. In this study, we aim to investigate the effect of microgravity on the acti...
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Article
mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans
MicroRNA (miRNA) regulates gene expression in many cellular events, yet functions of only a few miRNAs are known in C. elegans. We analyzed the function of mir-35-41 unique to the worm, and show here that mir-35 ...
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Article
Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system
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Article
Regulation of transcriptionally active genes via the catalytically inactive Cas9 in C. elegans and D. rerio
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Chapter and Conference Paper
Adaptive Fuzzy Control Algorithm for an Integrated Navigation of SINS and the Odometer
Accuracy is one of the most important requirements for land vehicle navigation. Since the accuracy of SINS degrades rapidly with time while that of odometer degrades with distance, a SINS/OD integrated navigat...
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Article
Open AccessRETRACTED ARTICLE: Up-regulation of CDK16 by multiple mechanisms in hepatocellular carcinoma promotes tumor progression
Hepatocellular carcinoma (HCC) remains difficult to cure due to lack of effective treatment and the molecular mechanisms are complex and not completely understood. In this study, We investigated the role of CD...
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Article
Open AccessUpregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway
Infection with the hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma (HCC). The osmoregulatory transcription factor nuclear factor of activated T-cells 5 (NFAT5) ha...
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Article
Open AccessOrthogonal Cas9–Cas9 chimeras provide a versatile platform for genome editing
The development of robust, versatile and accurate toolsets is critical to facilitate therapeutic genome editing applications. Here we establish RNA-programmable Cas9-Cas9 chimeras, in single- and dual-nuclease...
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Article
Open AccessNmeCas9 is an intrinsically high-fidelity genome-editing platform
The development of CRISPR genome editing has transformed biomedical research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus pyogenes SF370 (SpyCas9). With many RNA guides, wild...
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Article
Open AccessPublisher Correction: Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing
The original version of this Article contained errors in the author affiliations. Mehmet Fatih Bolukbasi was incorrectly associated with Bluebird Bio., Cambridge, MA, USA and Ankit Gupta was incorrectly associ...
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Article
Precise therapeutic gene correction by a simple nuclease-induced double-stranded break
Current programmable nuclease-based methods (for example, CRISPR–Cas9) for the precise correction of a disease-causing genetic mutation harness the homology-directed repair pathway. However, this repair proces...
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Article
Highly efficient therapeutic gene editing of human hematopoietic stem cells
Re-expression of the paralogous γ-globin genes (HBG1/2) could be a universal strategy to ameliorate the severe β-globin disorders sickle cell disease (SCD) and β-thalassemia by induction of fetal hemoglobin (HbF,...
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Article
Open AccessRetraction Note: Up-regulation of CDK16 by multiple mechanisms in hepatocellular carcinoma promotes tumor progression
The Editor-in-Chief has retracted this article [1] due to concerns about the figures. In Figure 3C, the images from the colony formation assays of all cell lines, excluding the control for the HCCLM9 cell line...
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Article
Experimental Investigation on Ejector Performance Using R134a as Refrigerant
Ejector refrigeration has the advantage of low capital cost, simple design, reliable operation, long lifespan and almost no maintenance. The only weakness is the low efficiency and its intolerance to deviation...
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Article
Cancer exosome-derived miR-9 and miR-181a promote the development of early-stage MDSCs via interfering with SOCS3 and PIAS3 respectively in breast cancer
We previously identified that the development of early-stage myeloid-derived suppressor cells (eMDSCs) in breast cancer with high IL-6 (IL-6high) expression was correlated with the SOCS3 deficiency-dependent hype...
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Article
LINC00662 promotes hepatocellular carcinoma progression via altering genomic methylation profiles
The identification of viability-associated long noncoding RNAs (lncRNAs) is a means of uncovering therapeutic approaches for hepatocellular carcinoma (HCC). In addition, aberrant genome-wide hypomethylation ha...
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Chapter and Conference Paper
Fast Mining of Top-k Frequent Balanced Association Rules
Association rule mining (ARM), as a basic data mining task, aims to find association rules that satisfy predefined parameters from a given database. However, traditional ARM algorithms always generate a huge n...
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Chapter and Conference Paper
A New Approach on Satellite Mission Planning with Revisiting Requirements
With the development of space science and technology, the demand of observation mission increases. However, due to the limitations of the performance of platform or payloads and space environment of the remote...
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Chapter and Conference Paper
Application of Population Based Incremental Learning Algorithm in Satellite Mission Planning
Considering the increasing demand for earth observation missions, aiming at the centralized cooperative mission planning problem of remote sensing satellites, analyzing the constraints in the operation of sate...
