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Article
Single-atom Pd catalysts as oxidase mimics with maximum atom utilization for colorimetric analysis
Pd-based nanomaterials have shown great promise as potential mimic enzymes, but conventional catalysts use only a small fraction of the Pd content that located on the catalyst’s surface. Herein, we demonstrate...
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Article
Colorimetric detection of acetylcholinesterase and its inhibitor based on thiol-regulated oxidase-like activity of 2D palladium square nanoplates on reduced graphene oxide
A convenient and sensitive colorimetric assay for acetylcholinesterase (AChE) and its inhibitor has been designed based on the oxidase-like activity of {100}-faceted Pd square nanoplates which are grown in sit...
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Article
Ultrathin PdCu alloy nanosheet–assembled 3D nanoflowers with high peroxidase-like activity toward colorimetric glucose detection
Enzyme-mimetic properties of nanomaterials can be efficiently tuned by controlling their size, composition, and structure. Here, ultrathin PdCu alloy nanosheet–assembled three-dimensional (3D) nanoflowers (Pd1Cux
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Article
Colorimetric determination of the activity of alkaline phosphatase by exploiting the oxidase-like activity of palladium cube@CeO2 core-shell nanoparticles
Core-shell palladium cube@CeO2 (Pd cube@CeO2) nanoparticles are shown to display oxidase-like activity. This is exploited in a method for determination of the activity of alkaline phosphatase (ALP). The Pd cube@C...
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Article
Fluorometric and colorimetric dual-readout alkaline phosphatase activity assay based on enzymatically induced formation of colored Au@Ag nanoparticles and an inner filter effect
An ultrasensitive fluorometric and colorimetric dual-mode assay is described for the determination of the activity of alkaline phosphatase (ALP). ALP catalyzes the decomposition of 2-phospho-L-ascorbic acid, a...
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Article
Spectrophotometric determination of the activity of alkaline phosphatase and detection of its inhibitors by exploiting the pyrophosphate-accelerated oxidase-like activity of nanoceria
The oxidase-like activity of nanoceria is low. This limits its practical applications. It is demonstrated here that pyrophosphate ion (PPi) can improve the oxidase-like activity of nanoceria. Specifically, nan...
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Article
Fluorometric determination of the activity of alkaline phosphatase and its inhibitors based on ascorbic acid-induced aggregation of carbon dots
The authors describe a fluorometric method for determination of the activity of alkaline phosphatase (ALP) and its inhibitors. Nitrogen and boron co-doped carbon dots (C-dots) with excitation/emission peaks at...
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Article
Fluorometric determination of sulfide ions via its inhibitory effect on the oxidation of thiamine by Cu(II) ions
A fluorometric assay is described for sulfide ions determination. It is based on the finding that the oxidation of the non-fluorescent substrate thiamine (TH) by Cu(II) in basic solution to form fluorescent th...
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Article
Colorimetric determination of the activity of acetylcholinesterase and its inhibitors by exploiting the iodide-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide
We describe a sensitive and selective colorimetric method for the determination of the activity of the enzyme acetylcholinesterase (AChE) and its inhibitors. Detection is based on the fact that acetylthiocholi...
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Article
Increased response to oxidative stress challenge of nano-copper-induced apoptosis in mesangial cells
Recently, many studies reported that nanosized copper particles (nano-Cu, the particle size was around 15–30 nm), one of the nanometer materials, could induce nephrotoxicity. To detect the effect of nano-Cu on...
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Article
In vitro toxicity of nanosized copper particles in PC12 cells induced by oxidative stress
Recent evidence suggests that some nanomaterials, which are widely used in many fields, have health effects. In order to investigate the cytotoxicity induced by nanosized copper particles (nano-Cu), PC12 cells...