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Open AccessNickel and low CO2-controlled motility in Chlamydomonasthrough complementation of a paralyzed flagella mutant with chemically regulated promoters
Chlamydomonas reinhardtii is a model system for the biology of unicellular green algae. Chemically regulated promoters, such as the nickel-inducible CYC6 or the low CO2-inducible CAH1 promoter, may prove useful f...
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Open AccessAgrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study
Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression o...
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Open AccessSILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species
The use of sequencing and genoty** platforms has undergone dramatic improvements, enabling the generation of a wealth of genomic information. Despite this progress, the availability of high-quality genomic D...
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Open AccessGeneration and physiological characterization of genome-edited Nicotiana benthamiana plants containing zeaxanthin as the only leaf xanthophyll
Simultaneous genome editing of the two homeologous LCYe and ZEP genes of Nicotiana benthamiana results in plants in which all xanthophylls are replaced by zeaxanthin.
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First report of pseudopithomyces mori causing leaf spot on mandarin in Italy
