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Chapter
Effect of Cell Line and Differentiation on the Oxygenation Status of Experimental Sarcomas
Since the measurement of tumor oxygenation status and the fraction of hypoxia within tumor tissue by computerized pO2-histography [10] has become increasingly im-portant in the clinical setting, many studies on t...
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Chapter
Tumor Oxygenation Under Normobaric and Hyperbaric Hyperoxia
Tumor hypoxia is an important factor limiting the efficiency of sparsely ionizing ra-diation and O2-dependent chemotherapy. Since the tumor pO2 is the result of a dynamic steady state between oxygen supply and O2
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Chapter
Current Status of Knowledge and Critical Issues in Tumor Oxygenation
In 1972 we started the systematic investigation of oxygenation status in (a) experimental tumor systems in rats and mice [e.g., 1–6], (b) in human tumors xenotransplanted into immune-deficient rnu/rnu-rats [e....
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Article
Localized hypothermia: impact on oxygenation, microregional perfusion, metabolic and bioenergetic status of subcutaneous rat tumours
The effect of localized hypothermia on microcirculatory and metabolic parameters in s.c. DS sarcomas on the hind foot dorsum of Sprague-Dawley rats was investigated. Tumours were cooled by superfusion of the t...
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Article
Enhanced radiosensitivity in experimental tumours following erythropoietin treatment of chemotherapy-induced anaemia
The radiosensitivity of solid tumours in anaemic rats treated with recombinant human erythropoietin (rhEPO, epoetin beta) was studied. Anaemia was induced by a single dose of carboplatin (45 mg kg(-1) i.v.), r...
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Article
Open AccessDisparate responses of tumour vessels to angiotensin II: tumour volume-dependent effects on perfusion and oxygenation
Perfusion and oxygenation of experimental tumours were studied during angiotensin II (AT II) administration whereby the rate of the continuous AT II infusion was chosen to increase the mean arterial blood pres...
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Article
Recombinant human erythropoietin increases the radiosensitivity of xenografted human tumours in anaemic nude mice
Purpose: The effect of recombinant human erythropoietin (Epo) on the radiosensitivity of human tumour xenografts growing in anaemic nude mice was studied. Methods and materials: Anaemia was induced by total body...
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Article
Normo- and hyperbaric oxygenation of tumors: from bench to bedside
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Article
Open AccessExpression pattern of the urokinase-plasminogen activator system in rat DS-sarcoma: Role of oxygenation status and tumour size
The urokinase plasminogen activator system plays a central role in malignant tumour progression. Both tumour hypoxia and enhancement of urokinase plasminogen activator, urokinase plasminogen activator-receptor...
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Article
Open AccessNifedipine improves blood flow and oxygen supply, but not steady-state oxygenation of tumours in perfusion pressure-controlled isolated limb perfusion
Isolated limb perfusion allows the direct application of therapeutic agents to a tumour-bearing extremity. The present study investigated whether the dihydropyridine-type Ca2+-channel blocker nifedipine could imp...
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Article
Open AccessEnhanced effects of aminolaevulinic acid-based photodynamic therapy through local hyperthermia in rat tumours
The possibility of enhancing aminolaevulinic acid (ALA)-based photodynamic therapy (PDT) by simultaneous application of localised hyperthermia (HT) was evaluated. Treatments of rat DS-sarcomas included: (i) co...
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Article
Open AccessCombined hyperthermia and chlorophyll-based photodynamic therapy: tumour growth and metabolic microenvironment
The effects of combined and simultaneously applied localised 43°C hyperthermia (HT) and an antivascular bacteriochlorophyll-serine-based photodynamic therapy (Bchl-ser-PDT) on tumour growth and several microen...
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Article
Open AccessMicroenvironmental adaptation of experimental tumours to chronic vs acute hypoxia
This study investigated long-term microenvironmental responses (oxygenation, perfusion, metabolic status, proliferation, vascular endothelial growth factor (VEGF) expression and vascularisation) to chronic hyp...
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Article
Demonstration of pulmonary β2-adrenergic receptor binding in vivo with [18F]fluoroethyl-fenoterol in a guinea pig model
The new β2 radioligand (R,R)(S,S) 5-(2-(2-[4-(2-[18F]fluoroethoxy)phenyl]-1-methylethylamino)-1-hydroxyethyl)-benzene-1,3-diol ([18F]FE-fenoterol; [18F]FEFE), a fluoroethylated derivative of racemic fenoterol, wa...
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Chapter and Conference Paper
Acidosis Promotes Metastasis Formation by Enhancing Tumor Cell Motility
The tumor microenvironment is characterized by hypoxia, acidosis as well as other metabolic and biochemical alterations. Its role in cancer progression is increasingly appreciated especially on invasive capaci...
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Chapter and Conference Paper
Impact of the Tumor Microenvironment on the Expression of Inflammatory Mediators in Cancer Cells
Hypoxia and extracellular acidosis are common features of solid malignant tumors. The aim of the study was to analyze whether these pathophysiological parameters affect the expression of inflammatory mediators...
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Chapter
Hypoxia-Related Tumor Acidosis Affects MicroRNA Expression Pattern in Prostate and Breast Tumor Cells
MicroRNAs (miRNAs) are small non-coding RNA sequences which are able to modulate the expression of many functional proteins. The expression level of miRNAs can be modulated by parameters of the tumor microenvi...
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Chapter
Expression of MicroRNAs in Fibroblasts and Macrophages Is Regulated by Hypoxia-Induced Extracellular Acidosis
Under pathological conditions like inflammation, ischemia or in solid tumors, parameters of the microenvironment like local oxygenation and extracellular pH show marked changes when compared to healthy tissue....
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Chapter
Extracellular Acidosis Regulates the Expression of Inflammatory Mediators in Rat Epithelial Cells
Acidification of the cellular microenvironment is found in different pathological states such as inflammation, ischemia and in solid tumors. It can affect cell function and phenotype, and by this aggravate the...
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Chapter
Functional Impact of Acidosis-Regulated MicroRNAs on the Migration and Adhesion of Tumor Cells
Tumor tissue shows special features in metabolism in contrast to healthy tissue. Besides a distinctive oxygen deficiency, tumors often show a reduced extracellular pH (acidosis) resulting from an intensified g...
