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  1. Article

    Open Access

    Recent insights from in vitro single-molecule studies into nucleosome structure and dynamics

    Eukaryotic DNA is tightly packed into a hierarchically ordered structure called chromatin in order to fit into the micron-scaled nucleus. The basic unit of chromatin is the nucleosome, which consists of a shor...

    Orkide Ordu, Alexandra Lusser, Nynke H. Dekker in Biophysical Reviews (2016)

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  2. Article

    Open Access

    Essential validation methods for E. coli strains created by chromosome engineering

    Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used ...

    Sriram Tiruvadi Krishnan, M. Charl Moolman… in Journal of Biological Engineering (2015)

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  3. No Access

    Article

    Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments

    Magnetic torque tweezers are used to directly measure the torsional stiffness of single molecules of bare DNA and RecA filaments.

    Jan Lipfert, Jacob W J Kerssemakers, Tessa Jager, Nynke H Dekker in Nature Methods (2010)