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Article
Real-time observation of DNA translocation by the type I restriction modification enzyme EcoR124I
Type I restriction enzymes bind sequence-specifically to unmodified DNA and subsequently pull the adjacent DNA toward themselves. Cleavage then occurs remotely from the recognition site. The mechanism by which...
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Article
Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments
Magnetic torque tweezers are used to directly measure the torsional stiffness of single molecules of bare DNA and RecA filaments.
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Article
Open AccessRecent insights from in vitro single-molecule studies into nucleosome structure and dynamics
Eukaryotic DNA is tightly packed into a hierarchically ordered structure called chromatin in order to fit into the micron-scaled nucleus. The basic unit of chromatin is the nucleosome, which consists of a shor...
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Protocol
Measuring In Vivo Protein Dynamics Throughout the Cell Cycle Using Microfluidics
Studying the dynamics of intracellular processes and investigating the interaction of individual macromolecules in live cells is one of the main objectives of cell biology. These macromolecules move, assemble,...