7 Result(s)
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Protocol
ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
Protein-protein interactions (PPIs) play fundamental roles in all cellular processes. Especially membrane proteins facilitate a range of important biological functions in stimuli perception, signalling, and tr...
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Protocol
2 in 1 Vectors Improve in Planta BiFC and FRET Analysis
Protein-protein interactions (PPIs) play vital roles in all subcellular processes, and a number of tools have been developed for their detection and analysis. Each method has its unique set of benefits and dra...
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Protocol
ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
Protein–protein interactions (PPIs) play fundamental roles in all cellular processes. Especially membrane proteins facilitate a range of important biological functions in stimuli perception, signaling, and tra...
-
Protocol
2in1 Vectors Improve In Planta BiFC and FRET Analyses
Protein–protein interactions (PPIs) play vital roles in all subcellular processes and a number of tools have been developed for their detection and analysis. Each method has its unique set of benefits and draw...
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Article
Arabidopsis response regulator 22 inhibits cytokinin-regulated gene transcription in vivo
Cytokinin signaling in Arabidopsis is carried out by a two-component system (TCS) multi-step phosphorelay mechanism that involves three different protein families: histidine kinases (AHKs), phosphotransfer protei...
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Article
An RLP23–SOBIR1–BAK1 complex mediates NLP-triggered immunity
Plants and animals employ innate immune systems to cope with microbial infection. Pattern-triggered immunity relies on the recognition of microbe-derived patterns by pattern recognition receptors (PRRs). Necro...
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Article
Open AccessScreening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry
Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protei...
