7 Result(s)
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Open AccessAutomated single-cell proteomics providing sufficient proteome depth to study complex biology beyond cell type classifications
The recent technological and computational advances in mass spectrometry-based single-cell proteomics have pushed the boundaries of sensitivity and throughput. However, reproducible quantification of thousands...
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Open AccessWorkflow enabling deepscale immunopeptidome, proteome, ubiquitylome, phosphoproteome, and acetylome analyses of sample-limited tissues
Serial multi-omic analysis of proteome, phosphoproteome, and acetylome provides insights into changes in protein expression, cell signaling, cross-talk and epigenetic pathways involved in disease pathology and...
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Open AccessRNF43 G659fs is an oncogenic colorectal cancer mutation and sensitizes tumor cells to PI3K/mTOR inhibition
The RNF43_p.G659fs mutation occurs frequently in colorectal cancer, but its function remains poorly understood and there are no specific therapies directed against this alteration. In this study, we find that RNF...
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Open AccessProteomics of protein trafficking by in vivo tissue-specific labeling
Conventional approaches to identify secreted factors that regulate homeostasis are limited in their abilities to identify the tissues/cells of origin and destination. We established a platform to identify secr...
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Open AccessDual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer
Driver genes with a mutually exclusive mutation pattern across tumor genomes are thought to have overlap** roles in tumorigenesis. In contrast, we show here that mutually exclusive prostate cancer driver alt...
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Open AccessRapid and deep-scale ubiquitylation profiling for biology and translational research
Protein ubiquitylation is involved in a plethora of cellular processes. While antibodies directed at ubiquitin remnants (K-ɛ-GG) have improved the ability to monitor ubiquitylation using mass spectrometry, met...
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Article
Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS
Lenalidomide is a highly effective treatment for myelodysplastic syndrome (MDS) with deletion of chromosome 5q (del(5q)). Here, we demonstrate that lenalidomide induces the ubiquitination of casein kinase 1A1 ...
