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Article
Open AccessThe role of extracellular vesicles in animal reproduction and diseases
Extracellular vesicles (EVs) are nanosized membrane-enclosed compartments that serve as messengers in cell-to-cell communication, both in normal physiology and in pathological conditions. EVs can transfer func...
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Article
Open AccessA classical swine fever virus E2 fusion protein produced in plants elicits a neutralizing humoral immune response in mice and pigs
Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated ...
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Open AccessReconstruction of bovine spermatozoa substances distribution and morphological differences between Holstein and Korean native cattle using three-dimensional refractive index tomography
Measurements of the three-dimensional (3D) structure of spermatozoon are crucial for the study of developmental biology and for the evaluation of in vitro fertilization. Here, we present 3D label-free imaging of ...
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Article
Open AccessFunctional roles of hnRNPA2/B1 regulated by METTL3 in mammalian embryonic development
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) plays an important role in RNA processing via in m6A modification of pre-mRNA or pre-miRNA. However, the functional role of and relationship between m6A ...
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Article
Open AccessAutophagy is required for proper meiosis of porcine oocytes maturing in vitro
Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine...
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Article
Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus
Plants are promising host systems for recombinant protein production. However, progress in the commercialization of plant-made proteins (PMPs) has been slow. Only one PMP drug is commercially available. In thi...
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Article
Open AccessC-Phycocyanin protects against mitochondrial dysfunction and oxidative stress in parthenogenetic porcine embryos
C-Phycocyanin (CP) is a biliprotein enriched in blue-green algae that is known to possess antioxidant, anti-apoptosis, anti-inflammatory, and radical-scavenging properties in somatic cells. However, the protec...
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Article
Open AccessMelatonin enhances the developmental competence of porcine somatic cell nuclear transfer embryos by preventing DNA damage induced by oxidative stress
Melatonin has antioxidant and scavenger effects in the cellular antioxidant system. This research investigated the protective effects and underlying mechanisms of melatonin action in porcine somatic cell nucle...
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Article
Open AccessThe possible molecular mechanisms of bisphenol A action on porcine early embryonic development
Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. ...
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Article
Production of transgenic chickens constitutively expressing human erythropoietin (hEPO): Problems with uncontrollable overexpression of hEPO gene
The chicken is a promising candidate as a bioreactor for the economical mass production of human therapeutic proteins. Here, we report the successful generation of transgenic chickens that produce high concent...
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Open AccessInhibition of Rac1 GTPase activity affects porcine oocyte maturation and early embryo development
Mammalian oocyte asymmetric division relies on the eccentric positioning of the spindle, resulting in the polar body formation. Small signaling G protein Rac1 is a member of GTPases, which regulates a diverse ...
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Article
Open AccessExposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes
T-2 toxin is a main type A trichothecene mycotoxin which is the most toxic trichothecence. T-2 toxin has posed various toxic effects on human and animals in vigorous cell proliferation tissues like lymphoid, h...
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Article
Open AccessStochastic anomaly of methylome but persistent SRY hypermethylation in disorder of sex development in canine somatic cell nuclear transfer
Somatic cell nuclear transfer (SCNT) provides an excellent model for studying epigenomic reprogramming during mammalian development. We mapped the whole genome and whole methylome for potential anomalies of mu...
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Article
Open AccessTropomodulin-3 is essential in asymmetric division during mouse oocyte maturation
The dynamic polymerization and depolymerization of actin filaments is essential for various cellular processes such as cell migration, rotation, cytokinesis, and mammalian oocyte maturation. Tropomodulin 3 (Tm...
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Article
MLL2 is essential for porcine embryo development in vitro
Several germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, their function during early embryo development has been poorly e...
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Article
Open AccessDepletion of the LINC complex disrupts cytoskeleton dynamics and meiotic resumption in mouse oocytes
The SUN (Sad-1/UNC-84) and KASH (Klarsicht/ANC-1/Syne/homology) proteins constitute the linker of nucleoskeleton and cytoskeleton (LINC) complex on the nuclear envelope. To date, the SUN1/KASH5 complex is know...
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Article
Open AccessStructural basis for recognition of Emi2 by Polo-like kinase 1 and development of peptidomimetics blocking oocyte maturation and fertilization
In a mammalian oocyte, completion of meiosis is suspended until fertilization by a sperm and the cell cycle is arrested by a biochemical activity called cytostatic factor (CSF). Emi2 is one of the CSFs and it ...
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Article
Zinc depletion activates porcine metaphase II oocytes independently of the protein kinase C pathway
Zinc is an important trace element that regulates several biological functions. This study investigated the role of zinc in the metaphase (M) II arrest of porcine oocytes. N, N, N’, N’-tetrakis(2-pyridylmethyl...
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Article
Production of transgenic spermatozoa by lentiviral transduction and transplantation of porcine spermatogonial stem cells
Spermatogonial stem cells (SSCs) are adult stem cells that transmit genetic information from the parent to the next generation (progeny) in males, and thus, SSCs have used in germline-modification for generati...
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Poly-lysine peptidomimetics having potent antimicrobial activity without hemolytic activity
Diversity of sequence and structure in naturally occurring antimicrobial peptides (AMPs) limits their intensive structure–activity relationship (SAR) study. In contrast, peptidomimetics have several advantages...
