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  1. No Access

    Article

    Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees

    A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in sho...

    M. C. San-José, E. Corredoira, M. T. Martínez, N. Vidal… in Plant Cell Reports (2010)

  2. No Access

    Article

    Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur

    A protocol was developed for genetic transformation of somatic embryos derived from juvenile and mature Quercus robur trees. Optimal transformation conditions were evaluated on the basis of the results of transie...

    N. Vidal, R. Mallón, S. Valladares, A. M. Meijomín, A. M. Vieitez in Plant Cell Reports (2010)

  3. No Access

    Article

    High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

    An efficient protocol for genetic transformation of somatic embryos of Quercus robur by selection in a temporary immersion system is reported. The transformation frequency was 5 times higher than achieved by conv...

    R. Mallón, A. M. Vieitez, N. Vidal in Plant Cell, Tissue and Organ Culture (PCTOC) (2013)

  4. No Access

    Article

    Overexpression of the chestnut CsTL1 gene coding for a thaumatin-like protein in somatic embryos of Quercus robur

    Somatic embryos of Quercus robur derived from mature and juvenile trees were transformed with the CsTL1 gene coding for an antifungal protein. Clumps of somatic embryos were inoculated with Agrobacterium tumefaci...

    R. Mallón, S. Valladares, E. Corredoira… in Plant Cell, Tissue and Organ Culture (PCTO… (2014)

  5. No Access

    Article

    A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

    A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five ...

    N. Vidal, B. Blanco, B. Cuenca in Plant Cell, Tissue and Organ Culture (PCTOC) (2015)

  6. No Access

    Article

    Micropropagation of axillary shoots of hybrid chestnut (Castanea sativa × C. crenata) in liquid medium in a continuous immersion system

    A protocol for culturing chestnut axillary shoots with the basal sections continuously immersed in liquid medium was developed and the influence of various parameters was assessed: explant type and size, suppo...

    B. Cuenca, C. Sánchez, A. Aldrey, B. Bogo… in Plant Cell, Tissue and Organ Culture (PCTO… (2017)

  7. No Access

    Article

    Temporary immersion systems to improve alder micropropagation

    A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l− 1 benzyladenine and 0.5 mg l− 1 ...

    M. C. San José, N. Blázquez, M. J. Cernadas… in Plant Cell, Tissue and Organ Culture (PCTO… (2020)