11 Result(s)
within
M.A. Machado
Life Sciences
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Article
Resistance of Citrus and Related Genera to Diaphorina citri Kuwayama (Hemiptera: Liviidae)
The present study was developed to evaluate the resistance of the following genotypes of Citrus and related genera to this pest: ‘Pera,’ ‘Natal’, and ‘Washington Navel’ oranges (Citrus sinensis), ‘Marsh Seedless’...
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Article
Detection of Brevipalpus-transmitted viruses in their mite vectors by RT–PCR
The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT–PCR of infected plant tissues. Here, we r...
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Article
In planta multiplication and graft transmission of ‘Candidatus Liberibacter asiaticus’ revealed by Real-Time PCR
The bacterium Candidatus Liberibacter asiaticus (CLas) is associated with huanglongbing (HLB) in citrus in many countries. Despite the fact that many characteristics of the disease are known, the rate of multipli...
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Article
Orchid fleck symptoms may be caused naturally by two different viruses transmitted by Brevipalpus
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee and several other plant species. There are two diffe...
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Article
Genetic diversity among nine species of Lippia (Verbenaceae) based on RAPD Markers
Nine species of Lippia (Verbenaceae) were studied by RAPD markers in order to evaluate the degree of genetic diversity. The following species were collected at the Cadeia do Espinhaço Mountains, Southeast Brazil:...
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Article
Assessment of genetic variability in grapefruits (Citrus paradisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers
The genetic variability of 38 grapefruit (Citrus paradisi Macf.) and three pummelos (C. maxima (Burm.) Merr..) accessions was evaluated using RAPD, and single sequence repeat (SSR) analyses. Approximately49% of t...
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Article
Genetic linkage maps of Citrus sunki Hort. ex. Tan. and Poncirus trifoliata (L.) Raf. and map** of citrus tristeza virus resistance gene
A linkage map for Citrus sunki Hort. ex. Tan. and Poncirus trifoliate (L.) Raf cv. Rubidoux has been developed exploiting the pseudo-testcross map** strategy and RAPD markers. From controlled crosses 314 F1 zyg...
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Article
Analysis of the genetic diversity among mandarins (Citrus spp.) using RAPD markers
RAPD markers were used to evaluate genetic similarity among 35 mandarin accessions, including 10 species and 7 hybrids. One octamer and twenty-two decamer primers produced 109 RAPDs, 45 of which were polymorph...
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Article
Morphological and molecular characterization of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop in Brazil
Brazilian isolates of Colletotrichum spp. from citrus orchards affected by postbloom fruit drop were examined for colony colour, mycelial growth, benomyl-resistance, pathogenicity, and genetic variability by r...
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Article
Genetic relationship of Mediterranean mandarins (Citrus deliciosa Tenore) using RAPD markers
Random amplified polymorphic DNA (RAPD) analysis was carried out to evaluate polymorphism and genetic similarity between 39 Mediterranean mandarin genotypes. One hundred eleven amplification products were iden...
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Article
A structural, functional and molecular analysis of plastids of the holoparasites Cuscuta reflexa and Cuscuta europaea
In the plastids of Cuscuta reflexa the number of thylakoids is strongly reduced compared with true chloroplasts and no typical grana are visible. The plastids of Cuscuta europaea lack thylakoids and the stroma is...
