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Article
Intraspecific and interspecific genetic and phylogenetic relationships in the genus Populus based on AFLP markers
Although Populus has become the model genus for molecular genetics and genomics research on forest trees, genetic and phylogenetic relationships within this genus have not yet been comprehensively studied at the ...
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Article
A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil
A reproducible Agrobacterium tumefaciens-mediated genetic transformation method that delivers fertile and morphologically normal transgenic plants was developed for cultivated tepary bean (Phaseolus acutifolius L...
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Chapter
Agrobacterium rhizogenes-Mediated Transformation of Plants
The soil bacterium Agrobacterium rhizogenes generates the “hairy root” syndrome in infected plants and is characterized by the neoplastic outgrowth of roots (Riker et al. 1930). The molecular basis of this phenom...
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Article
An optimized Agrobacterium-mediated transformation procedure for Phaseolus acutifolius A. Gray
To improve Agrobacterium tumefaciens-mediated transformation of Phaseolus acutifolius, we examined the effect of different factors on T-DNA transfer by measuring transient expression levels of an intron-containi...
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Article
Genetic differentiation among three neighboring Brazil-cherry (Eugenia uniflora L.) populations within the Brazilian Atlantic rain forest
Eugenia uniflora L. (pitanga) is widely distributed in tropical areas. It is present in coastal vegetation from Ceara, in northeastern Brazil, to Rio Grande do Sul, at the southern tip of the country. Eugenia un...
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Chapter
Metabolic Colonisation: the Fate of a Leafy Gall
The Gram-positive plant pathogen Rhodococcus fascians has a broad host range and induces malformations without killing its host (7). To provoke symptoms the bacteria require the action of virulence genes located ...
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Chapter
Small scale spatial genetic structure of six tropical tree species in French Guiana
The small scale spatial genetic structure of six tropical tree species (Carapa procera, Chrysophyllum sanguinolentum, Dicorynia guianensis, Eperua grandiflora, Virola michelii, Vouacapoua americana) was studied f...
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Article
Sudan-β-d-glucuronides and their use for the histochemical localization of β-glucuronidase activity in transgenic plants
Synthesis of five different Sudan-β-d-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-d-glu...
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Article
Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism
Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the...
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Article
Dual action of the active oxygen species during plant stress responses
Adaptation to environmental changes is crucial for plant growth and survival. However, the molecular and biochemical mechanisms of adaptation are still poorly understood and the signaling pathways involved re...
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Chapter
Molecular Aspects of Bud Dormancy in Trees
Dormancy is a survival strategy that enables plants or plant parts to persist periods that are unfavorable for growth. It assures a seasonally synchronized growth through seed dormancy in annual and perennial ...
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Chapter
Control of Lignin Biosynthesis
The ever increasing worldwide use of forest tree products, which coincides with the diminishing of natural forests, necessitates programs for efficient tree farming. For this purpose, there is a demand for acc...
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Article
Hairy root production in Arabidopsis thaliana: cotransformation with a promoter-trap vector results in complex T-DNA integration patterns
In comparison with the production of transgenic plants, the generation of hairy roots has the advantage that more independent transgenic lines can be produced in a shorter period of time. Therefore, we wanted...
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Article
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana
The higher plant Arabidopsis thaliana (Arabidopsis) is an important model for identifying plant genes and determining their function. To assist biological investigations and to define chromosome structure, a coor...
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Article
Comparison of PCR-based molecular marker analyses of Musa breeding populations
Progress in the breeding of plantain and banana has been restricted by the complex genetic structure and behaviour of cultivated polyploid Musa. Genetic improvement has been hindered due to the large amount of...
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Article
AFLP analysis of genetic diversity within and between Arabidopsis thaliana ecotypes
The degree of genetic diversity within and between 21 Arabidopsis thaliana (L.) Heynh ecotypes was estimated by AFLP analysis. Within seven of the 21 ecotypes, a low but significant level of polymorphism was det...
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Chapter
Agrobacterium-Mediated Transformation of Phaseolus Beans
The grain legumes are an important group of crop species, belonging to the family of the Fabaceae. They are grown for human and animal nutrition all over the world. Examples include soybean (Glycine max), peanut ...
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Chapter
Ethylene Signaling: More Players in the Game
The Arabidopsis ethylene response pathway was established by characterization of ethylene mutants that were isolated exploiting the triple response of dark-grown seedlings. The various triple response screens wer...
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Chapter
Integration of nematode-responsive regulatory sequences from Arabidopsis thaliana into nematode control strategies
While for many decades fundamental research on sedentary nematode-plant parasitism encompassed mainly the ultrastructural and physiological aspects of the interaction, more recently also the area of molecular ...
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Chapter
Identification of Novel Cell Cycle Genes in Arabidopsis Thaliana
The aim of our research is to understand the molecular basis of cell cycle control in higher plants, in casu in Arabidopsis thaliana, and how intrinsic developmental signals and environmental factors influence ce...
