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Introduction of pokeweed antiviral protein cDNA intoBrassica napus and acquisition of transgenic plants resistant to viruses
Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-inducible promoter has been introduced intoBrassica napus by coculture withAgrobacterium tumefaciens and laser microbeam puncture respecti...
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Article
A high-Tc dc-SQUID-based single-channel MCG and its performance
A single-channel high-Tc dc-SQUID magnetometer and gradiometer have been developed to record the magnetic field component perpendicular to the human chest generated by heart-beat. Magnetocardiogram (MCG) measu...
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Article
Rational groundwater table indicated by the eco-physiological parameters of the vegetation: A case study of ecological restoration in the lower reaches of the Tarim River
The eco-physiological response and adaptation of Populus euphratica Oliv and Tamarix ramosissima Ldb during water release period were investigated. Nine typical areas and forty-five tran-sects were selected along...
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Article
Open AccessPreparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum
Hierarchical imprinting was developed to prepare the protein imprinted materials, as the artificial antibody, for the selective depletion of HSA from the human serum proteome. Porcine serum albumin (PSA) was e...
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Article
RNA-seq profiling the transcriptome of secondary seed dormancy in canola (Brassica napus L.)
Secondary seed dormancy (SSD) is responsible for volunteer plants in canola fields, which causes a series of problems in canola production and serves as an important trait for the environmental safety assessme...
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Article
Open AccessReleasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation
Due to the important roles of N-glycoproteins in various biological processes, the global N-glycoproteome analysis has been paid much attention. However, by current strategies for N-glycoproteome profiling, pe...
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Article
Open AccessFlotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways
Lipid raft proteins have been confirmed to be important in cell signal transduction. Some reports have shown that the aberrant expression of lipid raft proteins is associated with malignant phenotypes in some ...
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Article
Open AccessEstablishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tob...
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Article
Open AccessConvergence of microbial assimilations of soil carbon, nitrogen, phosphorus and sulfur in terrestrial ecosystems
How soil microbes assimilate carbon-C, nitrogen-N, phosphorus-P and sulfur-S is fundamental for understanding nutrient cycling in terrestrial ecosystems. We compiled a global database of C, N, P and S concentr...
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Article
Open AccessNet-Immobilization of β-glucosidase on Nonwoven Fabrics to Lower the Cost of “Cellulosic Ethanol” and Increase Cellulose Conversions
The main limitation preventing the use of enzymatic cellulosic ethanol in industrial production is its higher cost which is mainly due to the elevated price of β-glucosidase (BG). Herein, we report on a simple...
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Article
Open AccessRapid evolution of tolerance to toxic Microcystis in two cladoceran grazers
Evolutionary adaptation could assist organisms to cope with environmental changes, yet few experimental systems allow us to directly track evolutionary trajectory. Using experimental evolution, evolutionary to...
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Article
Open AccessFast MS/MS acquisition without dynamic exclusion enables precise and accurate quantification of proteome by MS/MS fragment intensity
Most currently proteomic studies use data-dependent acquisition with dynamic exclusion to identify and quantify the peptides generated by the digestion of biological sample. Although dynamic exclusion permits ...
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Article
Open AccessEcosystem responses to warming and watering in typical and desert steppes
Global warming is projected to continue, leading to intense fluctuations in precipitation and heat waves and thereby affecting the productivity and the relevant biological processes of grassland ecosystems. He...
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Article
Open AccessQuantitative secretomic analysis of pancreatic cancer cells in serum-containing conditioned medium
Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies genera...
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Protein-imprinted material for the treatment of antibiotic-resistant bacteria
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Open AccessFormation of CuO nano-flowered surfaces via submerged photo-synthesis of crystallites and their antimicrobial activity
We report the fabrication of flower-like CuO nanostructured surfaces via submerged photo-synthesis of crystallites (SPSC), which requires only UV illumination in neutral water. In this paper, we discuss the react...
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Article
Open AccessInterfaces between hexagonal and cubic oxides and their structure alternatives
Multi-layer structure of functional materials often involves the integration of different crystalline phases. The film growth orientation thus frequently exhibits a transformation, owing to multiple possibilit...
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Article
Open AccessPhotochemistry and the role of light during the submerged photosynthesis of zinc oxide nanorods
Recently, metal oxide nanocrystallites have been synthesized through a new pathway, i.e., the submerged photosynthesis of crystallites (SPSC), and flower-like ZnO nanostructures have been successfully fabricat...
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Article
Open AccessMutation status and prognostic values of KRAS, NRAS, BRAF and PIK3CA in 353 Chinese colorectal cancer patients
Mutations in KRAS exon 2, BRAF and PIK3CA are commonly present in colorectal cancer (CRC) worldwide, but few data about RAS mutations outside KRAS exon 2 are available for Chinese CRCs. We, therefore, determined ...
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Open AccessTwo Contrasting Patterns and Underlying Genes for Coadaptation of Seed Dormancy and Flowering Time in Rice
Association between seed dormancy (SD) and flowering time (FT) may generate a synergy in plant adaptation. This research aimed to identify patterns and underlying genes of the association in rice (Oryza sativa). ...