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Article
Reduction of Benzaldehyde Catalyzed by Papain-Based Semisynthetic Enzymes
Some features of native enzyme’s active site were used to conjunction with a chemical reagent or modifying group, which would generate new functionality different from the natural enzyme. In order to obtain an...
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Article
Multicomponent Amorphous Nanofibers Electrospun from Hot Aqueous Solutions of a Poorly Soluble Drug
To design and fabricate multicomponent amorphous electrospun nanofibers for synergistically improving the dissolution rate and permeation profiles of poorly water-soluble drugs.
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Article
Kinetic Evaluation of Aminoethylisothiourea on Mushroom Tyrosinase Activity
This study demonstrates that aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase, is an irreversible competitive inhibitor of mushroom tyrosinase by chelation to the active site ...
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Article
Dissolution Improvement of Electrospun Nanofiber-Based Solid Dispersions for Acetaminophen
The objective of the present investigation was to prepare novel solid dispersions (SDs) of poorly water-soluble drugs with special microstructural characteristics using electrospinning process. With the hydrop...
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Article
In Vitro Effect of Ozagrel on Mushroom Tyrosinase
This investigation, in vitro, shows that ozagrel, an antithrombotic drug, inhibited both monophenolase and diphenolase activities of mushroom tyrosinase when l-tyrosine and l-DOPA were assayed spectrophotometrica...
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Article
The purification and characterization of deoxycytidine kinase from calf thymus
A facile and fast approach for the purification of deoxycytidine kinase (dCK) from calf thymus was developed using a fast performance liquid chromatography system. A 73-fold enrichment of the enzyme was noted ...
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Article
Lipase-catalyzed transesterification of soybean oil for biodiesel production in tert-amyl alcohol
Lipase-catalyzed transesterification of soybean oil and methanol for biodiesel production in tert-amyl alcohol was investigated. The effects of different organic medium, molar ratio of substrate, reaction tempera...
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Article
Enantioselective reductive amination of α-keto acids by papain-based semisynthetic enzyme
Alkylation of a cysteine residue in papain with a pyridoxamine (PX) cofactor was carried out. The resulting semisynthetic enzyme (papain-PX) has no detectable protease activity but has the ability to catalyze ...
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Article
Epoxypropane biosynthesis by Methylomonas sp. GYJ3: batch and continuous studies
Methylomonas sp. GYJ3 is a methanotrophic bacterium containing methane monooxygenase (MMO), which catalyses the epoxidation of propene to epoxypropane. In this study, the cell suspension of Methylomonas sp. GYJ3...