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Article
Open AccessA microbial supply chain for production of the anti-cancer drug vinblastine
Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary metabolites with many medicinal properties, including the essential anti-cancer therapeutics vinblastine and vincristine1. As MI...
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Article
Open AccessSuccession of physiological stages hallmarks the transcriptomic response of the fungus Aspergillus niger to lignocellulose
Understanding how fungi degrade lignocellulose is a cornerstone of improving renewables-based biotechnology, in particular for the production of hydrolytic enzymes. Considerable progress has been made in inves...
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Article
Open AccessIndustrial brewing yeast engineered for the production of primary flavor determinants in hopped beer
Flowers of the hop plant provide both bitterness and “hoppy” flavor to beer. Hops are, however, both a water and energy intensive crop and vary considerably in essential oil content, making it challenging to a...
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Article
Open AccessA bacterial pioneer produces cellulase complexes that persist through community succession
Cultivation of microbial consortia provides low-complexity communities that can serve as tractable models to understand community dynamics. Time-resolved metagenomics demonstrated that an aerobic cellulolytic ...
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Article
Oxidative cyclization of prodigiosin by an alkylglycerol monooxygenase-like enzyme
In Pseudoalteromonas rubra, an unclustered biosynthetic gene encodes a di-iron oxygenase-like enzyme that catalyzes regiospecific C–H activation and cyclization of prodigiosin, yet is unrelated to the Rieske oxyg...
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Article
Open AccessProgramming mRNA decay to modulate synthetic circuit resource allocation
Synthetic circuits embedded in host cells compete with cellular processes for limited intracellular resources. Here we show how funnelling of cellular resources, after global transcriptome degradation by the s...
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Article
Open AccessExpression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments
The capacity of fungi, such as Aspergillus niger, to degrade lignocellulose is harnessed in biotechnology to generate biofuels and high-value compounds from renewable feedstocks. Most feedstocks are currently pre...
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Article
Open AccessEngineering glucose metabolism of Escherichia coli under nitrogen starvation
A major aspect of microbial metabolic engineering is the development of chassis hosts that have favorable global metabolic phenotypes, and can be further engineered to produce a variety of compounds. In this w...
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Article
Open AccessMetabolic engineering for the high-yield production of isoprenoid-based C5 alcohols in E. coli
Branched five carbon (C5) alcohols are attractive targets for microbial production due to their desirable fuel properties and importance as platform chemicals. In this study, we engineered a heterologous isopreno...
