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Article
Open AccessDynamic movement of the Golgi unit and its glycosylation enzyme zones
Knowledge on the distribution and dynamics of glycosylation enzymes in the Golgi is essential for better understanding this modification. Here, using a combination of CRISPR/Cas9 knockin technology and super-r...
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Article
Open AccessCargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants
The trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different des...
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Article
Open AccessRole of Atg8 in the regulation of vacuolar membrane invagination
Cellular heat stress can cause damage, and significant changes, to a variety of cellular structures. When exposed to chronically high temperatures, yeast cells invaginate vacuolar membranes. In this study, we ...
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Article
Open AccessCOPII proteins exhibit distinct subdomains within each ER exit site for executing their functions
Secretory proteins are exported from special domains of the endoplasmic reticulum (ER) termed ER exit sites, via COPII-coated carriers. We recently showed that TANGO1 and Sec16 cooperatively organize mammalian...
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Article
Open AccessMiR-199a Inhibits Secondary Envelopment of Herpes Simplex Virus-1 Through the Downregulation of Cdc42-specific GTPase Activating Protein Localized in Golgi Apparatus
Because several studies have shown that exogenous miR-199a has antiviral effects against various viruses, including herpesviruses, we examined how miR-199a exerts its antiviral effects using epithelial tumour ...
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Article
Open AccessLive-cell visualization of excitation energy dynamics in chloroplast thylakoid structures
The intricate molecular processes underlying photosynthesis have long been studied using various analytic approaches. However, the three-dimensional (3D) dynamics of such photosynthetic processes remain unexpl...
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Article
Open AccessContact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER
Protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus is mediated by coat complex II (COPII) vesicles. It has been believed that COPII vesicles containing cargo are released from the ER ...