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Mass Spectrometric Identification of Proteins Isolated By Two-Dimensional Gel Electrophoresis
For twenty years two-dimensional polyacrylamide gel electrophoresis (2D PAGE) has been used to simultaneously separate and visualize by suitable staining, hundreds of proteins present in cell lysates [1, 2]. This...
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Article
Search of sequence databases with uninterpreted high-energy collision-induced dissociation spectra of peptides
We have broadened the utility of the SEQUEST computer algorithms to permit correlation of uninterpreted high-energy collision-induced dissociation spectra of peptides with all sequences in a database. SEQUEST ...