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Article
Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly
High-resolution structural studies are essential for understanding the folding and function of diverse RNAs. Herein, we present a nanoarchitectural engineering strategy for efficient structural determination o...
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Article
Reverse transcriptases lend a hand in splicing catalysis
The first high-resolution views of group II intron maturases illuminate the architectural and functional roles of these multidomain proteins in splicing and DNA invasion. The maturases show striking structural...
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Article
Evidence for a group II intron–like catalytic triplex in the spliceosome
It has long been thought that the catalytic RNAs of self-splicing group II introns and the spliceosome function by similar mechanisms. Now, a combination of genetic, cross-linking, and biochemical analyses of ...
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Article
A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination
Crystallization of RNA molecules can pose a challenge, and the U1A RNA binding protein has been used to facilitate the process. Now a different portable RNA sequence and a synthetic Fab are presented as tools ...
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Article
The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins
α-sarcin ribotoxins comprise a unique family of ribonucleases that cripple the ribosome by catalyzing endoribonucleolytic cleavage of ribosomal RNA at a specific location in the sarcin/ricin loop (SRL). The SR...
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Article
Metal ion coordination by the AGC triad in domain 5 contributes to group II intron catalysis
Group II introns require numerous divalent metal ions for folding and catalysis. However, because little information about individual metal ions exists, elucidating their ligands, functional roles and relation...
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Article
A new metal ion interaction in the Tetrahymena ribozyme reaction revealed by double sulfur substitution
The Tetrahymena ribozyme is a metalloenzyme that catalyzes cleavage of oligonucleotide substrates by phosphoryl transfer. Thiophilic metal ions such as Mn2+, Zn2+ or Cd2+ rescue the >103-fold inhibitory effect of...