9 Result(s)
within
Joo Won Suh
Biological Techniques
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Article
Elucidation of first step of the allylmalonyl-CoA biosynthetic pathway by expression of heterologous KSIII gene and enhancement of 36-methyl-FK506 production by genetic and chemical engineering
We identified another tcsABCD genes encoding biosynthesis of the allylmalonyl-CoA in new FK506 producing strain, Streptomyces sp. KCCM 11116P. The functionality of tcsABCD is confirmed by gene deletion. To valida...
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Article
Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage
Passalora fulva (or Fulvia fulva) is the causal microorganism of tomato leaf mold, the outbreak of which occurs worldwide in greenhouse especially when humidity is high. However, studies on antif...
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Article
Constituents of volatile compounds derived from Melaleuca alternifolia leaf oil and acaricidal toxicities against house dust mites
The acaricidal activities of the volatile compounds derived from Melaleuca alternifolia leaf oil were evaluated against house dust mites. Terpinen-4-ol (3.89 and 3.51 μg/cm2) was approximately 2.0 and 1.7 times m...
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Article
Isolation and characterization of anti-methicillinresistant Staphylococcus aureus/vancomycinresistant Enterococcus compound from Streptomyces bungoensis MJM 2077
Strain MJM2077 was selected for its strong anti-Methicillin-resistant Staphylococcus aureus/Vancomycin-resistant Enterococcus activity and designated as Streptomyces bungoensis MJM2077 based on the analysis of th...
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Article
Two base mutations of a putative LacI-family transcriptional regulator, SCO7554, reverse the overexpression effect on the antibiotic pigment production in Streptomyces coelicolor
The SCO7554 protein, a putative LacI-family transcriptional regulator, is important in the antibiotic pigment production in Streptomyces coelicolor M130. A single mutation at the 4th amino acid from asparagine to...
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Article
Effects of two putative LacI-family transcriptional regulators, SCO4158 and SCO7554, on antibiotic pigment production of Streptomyces coelicolor and Streptomyces lividans
Streptomycetes produce many secondary metabolites, including antibiotics, antifungal agents, anti-parasitic drugs, and immune suppressors. Evaluation of streptomycetes, Streptomyces coelicolor and Streptomyces li...
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Article
Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968 isolated from agricultural soil
MJM1968, an actinomycete showing potent antifungal activity, was isolated and identified by 16S rDNA sequence analysis as Streptomyces malaysiensis. MJM1968 showed strong antifungal activity on phytopathogenic fu...
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Article
PpsA, TufA, and glutaminase were modulated under Yop secretion conditions in Yersinia enterocolitica
Yersinia enterocolitica expresses the Ysc type III secretion system (TTSS) for delivery of effector proteins (Yops) to host cells to manipulate immune system of the host. The study on the physiol...
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Article
Accumulation of intracellular ATP is a factor suppressing morphological differentiation in adenosine kinase mutant of Streptomyces lividans
The present study demonstrated that the intracellular level of ATP was enhanced up to two-fold and the expression of ramR, a regulatory factor of aerial hyphae development, was repressed in an adenosine kinase mu...
