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Article
Fingerprinting cell lines: Use of human hypervariable DNA probes to characterize mammalian cell cultures
Hypervariable DNA sequences may be used as probes to derive DNA “fingerprints” for individuals. To assess the use of the human 33.15 and 33.6 probes (isolated by Jeffreys and coworkers) for characterizing cell.....
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Article
Analysis of large deletions in theHPRT gene of primary human fibroblasts using the polymerase chain reaction
Spontaneous and X-ray-induced mutants of theHPRT gene were isolated from two primary human fibroblast lines. The limited life-span of the mutants restricted the use of methods requiring large quantities of DNA, a...
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Article
The effectiveness of restriction endonucleases in cell killing and mutation
The use of restriction endonucleases (RE) to study the importance of DNA break end structures in differential cellular response has proved controversial. The number of DNA cut sites and the accessibility of RE...
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Article
Mutations induced by DNA double-strand breaks: The influence of genomic site
The transgenic CHO cell line PL61, carrying a recombinant SV40-gpt gene, was treated with restriction endonucleases to assess mutagenesis from defined DNA double-strand breaks. Mutations ingpt were measured under...
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Article
Chromosomal aberrations induced by defined DNA double-strand breaks: The origin of achromatic lesions
The mechanisms of formation of chromosomal aberrations are poorly understood, despite the common use of aberrations as a measure of the genetic effects of physical and chemical agents. We have used restriction...
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Article
Homologous recombination as a potential target for caffeine radiosensitization in mammalian cells: reduced caffeine radiosensitization in XRCC2 and XRCC3 mutants
The radiosensitizing effect of caffeine has been associated with the disruption of multiple DNA damage-responsive cell cycle checkpoints, but several lines of evidence also implicate inhibition of DNA repair. ...
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Article
Genetic interactions between RAD51 and its paralogues for centrosome fragmentation and ploidy control, independently of the sensitivity to genotoxic stresses
We evaluate here whether RAD51 and its paralogues XRCC2 and XRCC3 act via a common pathway for sensitivity to genotoxic stress, centrosome fragmentation and chromosome stability. We expressed the RAD51 dominan...