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  1. No Access

    Article

    Fingerprinting cell lines: Use of human hypervariable DNA probes to characterize mammalian cell cultures

    Hypervariable DNA sequences may be used as probes to derive DNA “fingerprints” for individuals. To assess the use of the human 33.15 and 33.6 probes (isolated by Jeffreys and coworkers) for characterizing cell.....

    John Thacker, Michael B. T. Webb, Paul G. Debenham in Somatic Cell and Molecular Genetics (1988)

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    Chapter

    Mechanisms of Resistance to Ionising Radiations: Genetic and Molecular Studies on Ataxia-Telangiectasia and Related Radiation-Sensitive Mutants

    In mammalian cells the mechanisms of resistance to damage caused by ionising radiations (IR) are largely unknown. Knowledge of the types of damage induced and of the enzymes, identified mostly in lower organis...

    John Thacker, Reg Wilkinson, Anil Ganesh… in DNA Repair Mechanisms and Their Biological… (1989)

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    Article

    Analysis of large deletions in theHPRT gene of primary human fibroblasts using the polymerase chain reaction

    Spontaneous and X-ray-induced mutants of theHPRT gene were isolated from two primary human fibroblast lines. The limited life-span of the mutants restricted the use of methods requiring large quantities of DNA, a...

    Tracy Morris, Walter Masson, Belinda Singleton… in Somatic Cell and Molecular Genetics (1993)

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    Article

    The effectiveness of restriction endonucleases in cell killing and mutation

    The use of restriction endonucleases (RE) to study the importance of DNA break end structures in differential cellular response has proved controversial. The number of DNA cut sites and the accessibility of RE...

    Nina D. Costa, Walter K. Masson, John Thacker in Somatic Cell and Molecular Genetics (1993)

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    Article

    Mutations induced by DNA double-strand breaks: The influence of genomic site

    The transgenic CHO cell line PL61, carrying a recombinant SV40-gpt gene, was treated with restriction endonucleases to assess mutagenesis from defined DNA double-strand breaks. Mutations ingpt were measured under...

    Nina D. Costa, John Thacker in Somatic Cell and Molecular Genetics (1996)

  6. No Access

    Article

    Chromosomal aberrations induced by defined DNA double-strand breaks: The origin of achromatic lesions

    The mechanisms of formation of chromosomal aberrations are poorly understood, despite the common use of aberrations as a measure of the genetic effects of physical and chemical agents. We have used restriction...

    Alison N. Harvey, Nina D. Costa, John R. K. Savage… in Somatic Cell and Molecular Genetics (1997)