128 Result(s)
within
Jianyu **
Life Sciences
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Article
In situ hybridization to metaphase chromosomes in six species ofPhaseolus andVigna using ribosomal DNA as the probe
In situ hybridization with a biotin-labeled rice ribosomal DNA (rDNA) probe to the somatic metaphase chromosomes of six species ofPhaseolus andVigna (P. angularis, P. calcaratus, P. coccineus, P. vulgaris, V. se...
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Article
Radiation-mediated proteolysis of CDT1 by CUL4–ROC1 and CSN complexes constitutes a new checkpoint
Genomic integrity is maintained by checkpoints that guard against undesired replication after DNA damage. Here, we show that CDT1, a licensing factor of the pre-replication complex (preRC)1,2,3, is rapidly proteo...
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Article
Study of the PTEN gene expression and FAK phosphorylation in human hepatocarcinoma tissues and cell lines
The tumor suppressor PTEN gene maps to chromosome 10q23.3 and encodes a dual specificity phosphatase. Mutations of this gene had been found in a variety of human tumors. In the present study, we analyzed the stru...
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Article
Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing
Introduction of exogenous DNA into mammalian cells represents a powerful approach for manipulating signal transduction. The available techniques, however, are limited by low transduction efficiency and low cel...
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Chapter and Conference Paper
Prediction of Protein Subcellular Localizations Using Moment Descriptors and Support Vector Machine
As more and more genomes have been discovered in recent years, it is an urgent need to develop a reliable method to predict protein subcellular localization for further function exploration. However many well-...
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Chapter and Conference Paper
Using Decision Templates to Predict Subcellular Localization of Protein
Theoretical and computational methods for the prediction of protein subcellular localization have been proposed and are develo** continuously. Many representations of protein sequence are proposed but a new ...
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Article
Active oxygen metabolism in the floral buds and leaves of the new cytoplasm male sterile (CMS) line and its maintainer line of non-heading Chinese cabbage
The cytoplasm male sterile (CMS) line and its maintainer line of the newly-bred non-heading Chinese cabbage were taken as experimental materials. The production rate of superoxide anion radical (O2 ...
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Article
Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates
Noscoc flagelliforme is a terrestrial macroscopic cyanobacterium with high economic value. Free-living cells that were separated from a natural colony of N. flagel...
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Article
Culture-independent nested PCR method reveals high diversity of actinobacteria associated with the marine sponges Hymeniacidon perleve and Sponge sp.
A culture-independent nested polymerase chain reaction (PCR) technique was used to investigate the diversity of actinobacteria communities associated with the sponges Hymeniacidon perleve and Sponge sp. The phylo...
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Article
Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin
5-Aminolevulinic acid synthase 1 (ALAS1) is the first and rate-controlling enzyme of heme biosynthesis. This study was to determine the effects of heme and selected nonheme metalloporphyrins on human ALAS1 gen...
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Article
Optimization and scale-up of a new photobleaching agar extraction process from Gracilaria lemaneiformis
An eco-friendly photobleaching extraction process for agar extraction from the red alga Gracilaria lemaneiformis was developed for the benefit of workers’ health and environmental safety. Here we report the optim...
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Article
Open AccessAberrant host immune response induced by highly virulent PRRSV identified by digital gene expression tag profiling
There was a large scale outbreak of the highly pathogenic porcine reproductive and respiratory syndrome (PRRS) in China and Vietnam during 2006 and 2007 that resulted in unusually high morbidity and mortality ...
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Article
An Electrophysiological Study of Acute Tetrodotoxin Poisoning
To evaluate the electrophysiological changes in patients with acute tetrodotoxin (TTX) poisoning from ingestion of globefish (Tetraodontidae) patients exposed to TTX were compared with age-matched controls. Th...
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Article
Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis
A total of 139 2-haloacid degrading bacteria strains were isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. After screening on indicator agar and ...
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Article
Association of ADAM33 gene with susceptibility to COPD in Tibetan population of China
Chronic obstructive pulmonary disease (COPD) is a common, complex disorder associated with substantial morbidity and mortality, influenced by both environmental factor and genetic factor. ADAM33 gene was found...
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Article
Differential expression of proteins in maize roots in response to abscisic acid and drought
Roots are highly sensitive organ in plant response to drought, which commonly inhibits root growth. However, less is known about the effect of ABA on root protein expression induced by drought. To help clarify...
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Article
Expression and function of two chaperone proteins, AtGroEL and AtGroES, from Acidithiobacillus ferrooxidans ATCC 23270
Two molecular chaperone genes encoding the Acidithiobacillus ferrooxidans Hsp60 (AtGroEL) and Hsp10 (AtGroES), respectively were introduced into Escherichia coli using the pLM1 expression vector. Then the AtGroEL...
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Article
Virus-Induced Gene Silencing of a Putative Glutathione S-Transferase Gene Compromised Ol-1-Mediated Resistance against Powdery Mildew in Tomato
Previously, cDNA-amplified fragment length polymorphism analysis was used to profile differentially expressed transcript-derived fragments (DE-TDFs) of tomato between resistant and susceptible responses agains...
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Article
Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors
Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a te...
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Article
Molecular cloning and functional characterization of a putative sulfite oxidase (SO) ortholog from Nicotiana benthamiana
Sulfite oxidase (SO) catalyzes the oxidation of sulfite to sulfate and thus has important roles in diverse metabolic processes. However, systematic molecular and functional investigations on the putative SO fr...
