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  1. No Access

    Article

    A New Method for the Detection of Amino-Acids, Peptides, Proteins and Other Buffering Substances on Paper

    DURING the past few years, a large number of papers have appeared dealing with the quantitative determination of amino-acids in paper chromatograms in situ or after elution; but no simple satisfactory general met...

    J. PORATH, P. FLODIN in Nature (1951)

  2. Article

    Structure of Bacitracin A

    A BACITRACIN polypeptide purified by charcoal chromatography was shown by ultracentrifugation to have a molecular weight of 1,400–1,5001. Recently, by a modified procedure using milder conditions, a peptide of hi...

    J. PORATH in Nature (1953)

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  3. No Access

    Article

    Migration-elution of Zones in an Electrophoresis Column

    SO far the preparation of electrophoretically defined fractions by means of zone electrophoresis in powder columns has been performed in two separate steps. Electrophoresis has been succeeded by displacement o...

    J. PORATH, E. B. LINDNER, S. JERSTEDT in Nature (1958)

  4. No Access

    Article

    Gel Filtration as a Method for Purification of Protein-bound Peptides Exemplified by Oxytocin and Vasopressin

    WE would like to call attention to a new procedure suitable for purification of simple compounds weakly associated with high molecular weight substances. The method, which was proposed elsewhere by one of us1, is...

    E. B. LINDNER, A. ELMQVIST, J. PORATH in Nature (1959)

  5. No Access

    Article

    A Method for concentrating Solutes of High Molecular Weight

    CONCENTRATION of diluted aqueous solutions of labile solutes of high molecular weight, such as proteins, is often difficult in the laboratory as well as on a technical scale. We wish to present a mild concentr...

    PER FLODIN, B. GELOTTE, J. PORATH in Nature (1960)

  6. No Access

    Article

    Separation Methods based on Molecular Sieving and Ion Exclusion

    CROSS-LINKED dextran (‘Sephadex’) has been shown to possess properties of a molecular sieve1. The size limits for effective molecular sieving depend on the degree of cross-linking. Molecules or ions capable of en...

    J. PORATH, E. B. LINDNER in Nature (1961)

  7. No Access

    Article

    Sweden: Molecular Sieving and Adsorption

    Research and development in universities and industry in Sweden has provided methods for separating molecules according to their size, shape and sometimes charge by the molecular sieving effect of gel-forming ...

    J. PORATH in Nature (1968)