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Article
Improvement of Slee** Beauty Transposon System Enabling Efficient and Stable Protein Production
The most essential goal in the field of biopharmaceuticals is to develop cell lines with higher protein yields. To this goal, the Slee** Beauty (SB) transposon-based expression system has been developed as a...
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Article
Bacterial Artificial Chromosome-based Protein Expression Platform Using the Tol2 Transposon System
Conventional vector systems, including plasmid-based vectors, are mainly used in mammalian cells for the production of biopharmaceuticals. Plasmid-based vectors express transgene by the random integration of r...
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Article
Rapid and Efficient BAC Recombineering: Gain & Loss Screening System
Recombineering has been developed to modify bacterial artificial chromosome (BAC) via homologous recombination. Nevertheless, as a screening strategy to identify the correct clone was not properly developed, i...
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Article
Cre/Lox-based RMCE for Site-specific Integration in CHO Cells
Traditional cell line development is based on random genomic integration of transgenes. Random integration leads to unpredictable expression and results in clonal heterogeneity requiring a tedious screening pr...
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Article
Oxygen permeation and oxidative coupling of methane with NiFe2O4-Gd0.1Ce0.9O2-δ composite membrane
Oxygen permeability and oxidative coupling of methane (OCM) characteristics of a 30 vol.% NiFe2O4-70 vol.% Gd0.1Ce0.9O2-δ (NFO-GDC) composite membrane were systematically investigated by varying operational condi...
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Article
Validation of analytical methods for organochlorine pesticide detection in shellfish and cephalopods by GC–MS/MS
This study validated the analysis of organochlorine pesticides (OCPs) in shellfish and cephalopods using a gas chromatograph equipped with a mass spectrometry (GC–MS/MS), and monitored residual pesticide level...
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Article
Comparison of acrylamide and furan concentrations, antioxidant activities, and volatile profiles in cold or hot brew coffees
The aim of this study was to investigate the formation of furan and acrylamide and to compare antioxidant capacities and volatile compounds in cold or hot brewed coffees. Cold brews were prepared at 5 °C and 2...
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Article
Migration study of caprolactam from polyamide 6 sheets into food simulants
Caprolactam, used in manufacturing polyamide (PA) 6, may threaten human health. Here, PA 6 sheets were produced by using a twin-screw extruder to evaluate its safety. Caprolactam migration concentrations from ...
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Article
Green lipped mussel oil complex suppresses lipopolysaccharide stimulated inflammation via regulating nuclear factor-κB and mitogen activated protein kinases signaling in RAW264.7 murine macrophages
Here we investigated the effect of green lipped mussel oil complex (GLMOC) on inflammation and underlying mechanism in lipopolysaccharide stimulated RAW264.7 murine macrophage cells. GLMOC containing green li...
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Article
Physicochemical properties of enzyme-treated waxy rice flour and expansion properties of Gangjung (a traditional Korean oil-puffed rice snack)
Long stee** process of waxy rice involved in Gangjung production is essential for obtaining good quality Gangjung, but it causes many related problems. The objective was to examine physiochemical properties of ...
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Article
Chiisanenside, a new triterpene glycoside from the fruits of Acanthopanax chiisanensis
A new triterpene glycoside was isolated from the fruits of Acanthopanax chiisanensis and identified as 3-O-β-d-[(6-carboxymethyl)-glucopyranosyl-(1 → 3)-β-d-glucuronopyranosyl]-olean-12-en-28-oic acid by spectral...
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Article
Application of Thermo-luminescence (TL) Method for the Identification of Food Mixtures Containing Irradiated Ingredients
Thermo-luminescence (TL) method was employed to identify irradiated garlic or ginger powder mixed at different proportions into the food base. The identification of irradiation was carried out by analyzing the...
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Article
Characterization and in Vitro Inhibition Studies of Bacillus anthracis FtsZ: A Potential Antibacterial Target
FtsZ is an essential bacterial cell division protein that is an attractive target for the development of antibacterial agents. FtsZ is a homologue of eukaryotic tubulin, has GTPase activity, and forms a ring-t...
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Article
Estrogen-like activity of Adenophora triphylla var. japonica water extract in MCF-7 cells
This study investigated the estrogen-like activity of Adenophora triphylla var. japonica, which has been shown to have pharmacological activities. Water extracts from A. triphylla (ATWE) could bind to estrogen re...
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Article
Sensory characteristics and cross-cultural comparisons of consumer acceptability for Gochujang dressing
This study was conducted to identify the sensory profiles of gochujang dressing prepared with different formulations, and to compare hedonic responses crossculturally. Gochujang dressing samples were prepared by ...
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Article
Tolerance of Saccharomyces cerevisiae K35 to lignocellulose-derived inhibitory compounds
The hydrolysis which converts polysaccharides to the fermentable sugars for yeast’s lingocellulosic ethanol production also generates byproducts which inhibit the ethanol production. To investigate the extent ...
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Article
Preparation of multifunctional polymeric micelles for antiviral treatment
In this study, a poly(L-lactic acid)-b-poly(ethylene glycol) (PLLA-b-PEG) diblock copolymer modified with a sialic acid derivative (methyl-b-neuraminic acid: mNA) was prepared for the development and application ...
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Article
Paclitaxel-polyurethane film for anti-cancer drug delivery: Film characterization and preliminary in vivo study
Polyurethane (PU) films incorporated with an anti-cancer drug paclitaxel (PTX) were prepared using a solvent casting method for potential applications to stent-based drug delivery and the local treatment of ma...
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Article
Development of glucose sensor using two-photon adsorbed photopolymerization
A novel glucose sensor was constructed, and its analytical potential examined. A chip-type three-electrode system for use in a flow-type electrochemical glucose sensor was fabricated using a UV lithography tec...
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Article
Anti-Helicobacter pylori diarylheptanoid identified in the rhizome of Alpinia officinarum
The antibacterial activity of materials derived from the rhizome of Alpinia officinarum (Zingiberaceae) against Helicobacter pylori ATCC 43504, ATCC 700392, and ATCC 700824 was examined using paper-disc diffusion...