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  1. No Access

    Article

    γ-Glutamyltranspeptidase-positive (GGT+) hepatocytes from carcinogen-treated rats isolated by magnetic beads

    Yi-hua Xu, Gerald L. Sattler… in In Vitro Cellular & Developmental Biology … (1991)

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    Article

    The bicarbonate ion is essential for efficient DNA synthesis by primary cultured rat hepatocytes

    Bicarbonate in the culture medium is essential for DNA synthesis of primary cultured rat hepatocytes stimulated by epidermal growth factor (EGF). When primary cultured hepatocytes in supplemented Leibovitz L15...

    Toshihiro Mitaka, Gerald L. Sattler… in In Vitro Cellular & Developmental Biology … (1991)

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    Article

    A method for the comparative study of replicative DNA synthesis in GGT-positive and GGT-negative hepatocytes in primary culture isolated from carcinogen-treated rats

    The presence of gamma-glutamyl transpeptidase (GGT) in focal nodules of hepatocytes is a commonly used marker for the identification of preneoplastic cell populations. Female Fischer 344 rats were initiated wi...

    Yi-Hua Xu, Gerald L. Sattler, Henry C. Pitot in In Vitro Cellular & Developmental Biology (1988)

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    Article

    Effects of extracellular matrix components on the growth and differentiation of cultured rat hepatocytes

    Some effects of culturing adult rat hepatocytes on each of four different substrates—laminin (LN), collagen type I (C-I), collagen type IV (C-IV), and fibronectin (FN)—have been investigated under defined cond...

    Norimasa Sawada, Akito Tomomura… in In Vitro Cellular & Developmental Biology (1987)

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    Article

    Use of a low-speed, iso-density percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations

    A simple yet effective method (iso-density percoll centrifugation) has been developed for consistently preparing isolated rat liver parenchymal cells with over 98% initial viability. The method has been applie...

    Bill L. Kreamer, Jeffrey L. Staecker… in In Vitro Cellular & Developmental Biology (1986)